FGF1 Protein (AA 1-155) (His tag,GST tag)
Quick Overview for FGF1 Protein (AA 1-155) (His tag,GST tag) (ABIN6239784)
抗原
See all FGF1 蛋白生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 1-155
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标记
- This FGF1 protein is labelled with His tag,GST tag.
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产品特性
- Tag location: N-terminal His and GST Tag
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Biological Activity Comment
- FGF1 (Fibroblast growth factor 1) is a member of FGF family, which plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. FGF1 is thought to stimulate the proliferation of 3T3 fibroblasts, besides, human FGF1 and mouse FGF1 shares similarities in amino acid sequence with the identity of 96%. Thus, a cell proliferation assay was conducted to detect the bioactivity of recombinant human FGF1 using 3T3 fibroblasts. Briefly, 3T3 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of FGF1. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of 3T3 cells after incubation with FGF1 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant FGF1 for 48h. The result was shown in Figure 2. It was obvious that FGF1 significantly increased cell viability of 3T3 cells.
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Fibroblast Growth Factor 1 (Acidic) (FGF1) (AA 16-155) (Active) protein (His tag)
FGF1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） > 97 % AcA, CC Active
Fibroblast Growth Factor 1 (Acidic) (FGF1) (Active) proteinFGF1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Fibroblast Growth Factor 1 (Acidic) (FGF1) (AA 16-155) protein (His tag)FGF1 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Fibroblast Growth Factor 1 (Acidic) (FGF1) (AA 16-155) protein (His tag)FGF1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Fibroblast Growth Factor 1 (Acidic) (FGF1) (AA 46-155) protein (GST tag)FGF1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Fibroblast Growth Factor 1 (Acidic) (FGF1) (AA 1-155) protein (GST tag)FGF1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Fibroblast Growth Factor 1 (Acidic) (FGF1) proteinFGF1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 97,00% SDS
Fibroblast Growth Factor 1 (Acidic) (FGF1) proteinFGF1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 97,00% SDS
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应用备注
- Isoelectric Point: 6.5
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
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储存液
- Dithiothreitol (DTT), Other preservative, ProClin
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注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))
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别名
- Fibroblast Growth Factor 1, Acidic (FGF1)
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背景
- Alternative Names: ECGF, AFGF, ECGFA, ECGFB, FGF-Alpha, FGFA, HBGF1, ECGFB, HBGF1, Acidic Fibroblast Growth Factor, heparin-binding growth factor 1, Endothelial Cell Growth Factor, Beta
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分子量
- 55kDa
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UniProt
- P05230
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途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
抗原
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