IL17D Protein (AA 35-169) (His tag,GST tag)
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- 抗原 See all IL17D 蛋白
- IL17D (Interleukin 17D (IL17D))
- 生物活性
- Active
- 产品特性
- AA 35-169
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This IL17D protein is labelled with His tag,GST tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His and GST Tag
- 纯度
- > 90 %
- Biological Activity Comment
- IL17D (Interleukin-17D) belongs to the IL17 (interleukin 17) family, which includes six members (IL-17, IL-17B through IL-17F). IL17D is reported to induce the expression of IL6, CXCL8/IL8, and CSF2/GM-CSF from endothelial cells. IL17 family members share some similarities in AA sequence and structure, thus, a binding ELISA assay was constructed to detect the association of recombinant human IL17D with recombinant human IL17RB. Briefly, IL17D were diluted serially in PBS with 0.1%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL17RB-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL17D pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of IL17D with IL17RB was shown in Figure 1 and this effect was in a dose dependent manner The binding activity of IL17D with IL17RA.
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- Discover our top product IL17D 蛋白
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Interleukin 17D (IL17D) (Active) protein
IL17D 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Interleukin 17D (IL17D) (AA 18-202) protein (His tag)IL17D 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 17D (IL17D) (AA 1-202) protein (GST tag)IL17D 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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- 应用备注
- Isoelectric Point: 9.1
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- IL17D (Interleukin 17D (IL17D))
- Abstract
- IL17D 产品
- 别名
- IL-17D Protein, IL-22 Protein, IL-27 Protein, IL27 Protein, AI462269 Protein, Il27a Protein, il17-4 Protein, zgc:194257 Protein, zgc:194303 Protein, IL-27p28 Protein, Il30 Protein, p28 Protein, interleukin 17D Protein, interleukin 17d Protein, interleukin 27 Protein, IL17D Protein, Il17d Protein, il17d Protein, Il27 Protein
- 背景
- Alternative Names: Interleukin-27
- 分子量
- 45kDa
- UniProt
- Q8TAD2
- 途径
- Cellular Response to Molecule of Bacterial Origin
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