Nerve Growth Factor Protein (NGF) (AA 19-239) (His tag)
NGF
宿主: 人
宿主: 大肠杆菌（E. Coli）
> 97 %
AcA, CC
Active
1 image
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- 抗原 See all Nerve Growth Factor (NGF) 蛋白
- Nerve Growth Factor (NGF)
- 生物活性
- Active
- 产品特性
- AA 19-239
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This Nerve Growth Factor protein is labelled with His tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 97 %
- Biological Activity Comment
- Nerve growth factor (NGF) is a neurotrophic factor and neuropeptide primarily involved in the regulation of growth, maintenance, proliferation, and survival of certain target neurons. As reported, when the pheochromocytoma cell line PC12 is exposed to nerve growth factor (NGF), the cells respond over a period of a week by ceasing cell division and extending neurites (Greene and Tischler, 1976). The cells were grown in Ham's F12K containing 5% fetal calf serum and 10% horse serum on polylysine or collagen coated plates. When cells reached log phase growth , fresh medium was added together with 10ng/mL of NGF, then cells were observed by inverted microscope everyday. Cell division ceasing and differentiation of PC12 cells after incubation with NGF (10ng/mL) for 6 days was shown in Figure1.Control group which received no NGF displayed no neurite outgrowth and cells multiply rapidly.
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- Discover our top product NGF 蛋白
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Nerve Growth Factor (NGF) (AA 122-239) protein (His tag)
NGF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Nerve Growth Factor (NGF) (AA 200-299) protein (GST tag)NGF 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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- 应用备注
- Isoelectric Point: 10.1
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- Nerve Growth Factor (NGF)
- Abstract
- NGF 产品
- 别名
- Beta-NGF Protein, HSAN5 Protein, NGFB Protein, Ngfb Protein, ngfb Protein, ns:zft386 Protein, xx:zft386 Protein, zgc:109730 Protein, beta-NGF Protein, hsan5 Protein, beta-ngf Protein, NGF Protein, ngf Protein, nerve growth factor Protein, nerve growth factor b (beta polypeptide) Protein, nerve growth factor S homeolog Protein, NGF Protein, Ngf Protein, ngfb Protein, ngf Protein, ngf.S Protein
- 背景
- Alternative Names: NGFB, Beta-NGF, HSAN5, NGF-B, Beta-Nerve Growth Factor
- 分子量
- 30/33kDa
- UniProt
- P22894
- 途径
- Regulation of Cell Size
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