Western Blotting (WB), SDS-PAGE (SDS), ELISA, Mass Spectrometry (MS)
原理
Recombinant Human OSTN Protein is produced by E.coli expression system and the target gene encoding Val28-Gly133 is expressed with a 6His tag at the N-terminus.
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
状态
Lyophilized
缓冲液
Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 7.5.
注意事项
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
储存条件
-20 °C
储存方法
Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.