Acbp, also known as Acyl-CoA-binding protein isoform 2, is a diazepam binding inhibitor, a protein that is regulated by hormones and is involved in lipid metabolism and the displacement of beta-carbolines and benzodiazepines, which modulatesignal transduction at type A gamma-aminobutyric acid receptors located in brain synapses. Expression of Acbp is significantly induced during adipocyte differentiation. Acbp acts as an acyl-CoA pool former and regulates long-chain fatty acids (LCFA) metabolism in peripheral tissues. Recombinant mouse Acbp protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.