SPR Protein (AA 1-262) (His tag)
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- 抗原 See all SPR 蛋白
- SPR (Sepiapterin Reductase (SPR))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-262
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宿主
- 小鼠
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资源
- 大肠杆菌（E. Coli）
- 标记
- This SPR protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MGSSHHHHHH SSGLVPRGSH MGSMEAGGLG CAVCVLTGAS RGFGRALAPQ LARLLSPGSV MLVSARSESM LRQLKEELGA QQPDLKVVLA AADLGTEAGV QRLLSAVREL PRPEGLQRLL LINNAATLGD VSKGFLNVND LAEVNNYWAL NLTSMLCLTS GTLNAFQDSP GLSKTVVNIS SLCALQPYKG WGLYCAGKAA RDMLYQVLAA EEPSVRVLSY APGPLDNDMQ QLARETSKDP ELRSKLQKLK SDGALVDCGT SAQKLLGLLQ KDTFQSGAHV DFYDC
- 纯度
- > 95 % by SDS - PAGE
- Top Product
- Discover our top product SPR 蛋白
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Sepiapterin Reductase (SPR) (AA 1-261) protein (Strep Tag)
Crystallography grade SPR 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Sepiapterin Reductase (SPR) (AA 1-261) protein (GST tag)SPR 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Sepiapterin Reductase (SPR) (AA 164-260) protein (GST tag)SPR 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Sepiapterin Reductase (SPR) (AA 1-261) protein (Strep Tag)Crystallography grade SPR 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Sepiapterin Reductase (SPR) protein (Myc-DYKDDDDK Tag)SPR 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.5) containing 1 mM DTT, 10 % glycerol
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- 抗原
- SPR (Sepiapterin Reductase (SPR))
- 别名
- Spr (SPR 产品)
- 别名
- SDR38C1 Protein, AA409688 Protein, ENSMUSG00000071345 Protein, Gm10328 Protein, CG12117 Protein, Dmel\\CG12117 Protein, SR Protein, sptr Protein, xspr Protein, DDBDRAFT_0188686 Protein, DDBDRAFT_0191275 Protein, DDB_0188686 Protein, DDB_0191275 Protein, zgc:194446 Protein, sepiapterin reductase Protein, Sepiapterin reductase Protein, sepiapterin reductase L homeolog Protein, sepiapterin reductase b Protein, SPR Protein, Spr Protein, Sptr Protein, spr.L Protein, sprE Protein, CpipJ_CPIJ000511 Protein, sr Protein, spr Protein, sprb Protein
- 背景
- Spr also known as Sepiapterin reductase, belongs to the Short-chain dehydrogenase/reductase (Sdr) family and also reduces various exogenous carbonyl compounds including phenylpropanedione. Spr is an essential enzyme for the biosynthesis of tetrahydrobiopterin, an essential cofactor for aromatic amino acid hydrolases including tyrosine hydroxylase, the rate-limiting enzyme in dopamine synthesis. Defects in Spr cause DOPA-responsive dystonia defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Recombinant mouse Spr protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
- 分子量
- 30.3kDa (285aa), confirmed by MALDI-TOF
- NCBI登录号
- NP_035597
- 途径
- Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour, Smooth Muscle Cell Migration
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