DCT Protein (AA 24-472) (His tag)
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- 抗原 See all DCT 蛋白
- DCT (Dopachrome Tautomerase (DCT))
- 蛋白类型
- Recombinant
- 产品特性
- AA 24-472
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宿主
- 人
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资源
- Baculovirus infected Insect Cells
- 标记
- This DCT protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- QFPRVCMTVD SLVNKECCPR LGAESANVCG SQQGRGQCTE VRADTRPWSG PYILRNQDDR ELWPRKFFHR TCKCTGNFAG YNCGDCKFGW TGPNCERKKP PVIRQNIHSL SPQEREQFLG ALDLAKKRVH PDYVITTQHW LGLLGPNGTQ PQFANCSVYD FFVWLHYYSV RDTLLGPGRP YRAIDFSHQG PAFVTWHRYH LLCLERDLQR LIGNESFALP YWNFATGRNE CDVCTDQLFG AARPDDPTLI SRNSRFSSWE TVCDSLDDYN HLVTLCNGTY EGLLRRNQMG RNSMKLPTLK DIRDCLSLQK FDNPPFFQNS TFSFRNALEG FDKADGTLDS QVMSLHNLVH SFLNGTNALP HSAANDPIFV VLHSFTDAIF DEWMKRFNPP ADAWPQELAP IGHNRMYNMV PFFPPVTNEE LFLTSDQLGY SYAIDLPVSV EETPGWPTTH HHHHH
- 纯度
- > 90 % by SDS - PAGE
- 内毒素水平
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Top Product
- Discover our top product DCT 蛋白
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.25 mg/mL
- 缓冲液
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- 抗原
- DCT (Dopachrome Tautomerase (DCT))
- 别名
- DCT (DCT 产品)
- 别名
- TYRP2 Protein, RGD1564975 Protein, trp2 Protein, DT Protein, TRP-2 Protein, TRP2 Protein, Tyrp-2 Protein, Tyrp2 Protein, slaty Protein, slt Protein, zgc:109860 Protein, dopachrome tautomerase Protein, dopachrome tautomerase L homeolog Protein, DCT Protein, Dct Protein, dct Protein, dct.L Protein
- 背景
- DCT, as known as L-dopachrome tautomerase isoform 1, is the tyrosinase family members. This protein plays a key role in the synthesis of the melanin pigment including tyrosinase (Tyr), tyrosinase-related protein 1 (Tyrp1). These are strongly implicated in eumelanin synthesis. The switch between the eumelanin and the pheomelanin pathways is thought to depend on the presence of cysteine. Thus, in the absence of cysteine, dopaquinone, the product of tyrosinase action, is transformed to cyclodopa (leucodopachrome) and then to dopachrome (and DOPA). Recombinant human DCT, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- 分子量
- 52.1kDa (455aa) 50-70kDa (SDS-PAGE under reducing conditions.)
- NCBI登录号
- NP_001913
- UniProt
- P40126
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