Carboxylesterase 2E (CES2E) (AA 27-559) (Active) protein (His tag)
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- 抗原 See all Carboxylesterase 2E (CES2E) 蛋白
- Carboxylesterase 2E (CES2E)
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 27-559
- 宿主
- 小鼠
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资源
- Baculovirus infected Insect Cells
- 标记
- His tag
- 应用范围
- SDS-PAGE (SDS)
- 序列
- QDSASPIRNT HTGQVRGSLV HVKDTDIAVH TFLGIPFAKP PVGPLRFAPP EAPEPWSGVR DGTSHPNMCL QNDNLMGSED LKMMNLILPP ISMSEDCLYL NIYVPAHAHE GSNLPVMVWI HGGALTVGMA SMYDGSMLAA TEDVVVVAIQ YRLGVLGFFS TGDQHAKGNW GYLDQVAALR WVQQNIVHFG GNPDRVTIFG ESAGGTSVSS HVVSPMSQGL FHGAIMESGV AVLPDLISSS SEMVHRIVAN LSGCAAVNSE TLMCCLRGKN EAEMLAINKV FKIIPGVVDG EFLPKHPQEL MASKDFHPVP SIIGINNDEY GWILPTIMDP AQKIEEITRK TLPAVLKSTA LKMMLPPECG DLLMEEYMGD TEDPETLQAQ FREMKGDFMF VIPALQVAHF QRSHAPVYFY EFQHRPSFFK DFRPPYVKAD HGDEIFLVFG YQFGNIKLPY TEEEEQLSRR IMKYWANFAR HGNPNSEGLP YWPVMDHDEQ YLQLDIQPSV GRALKARRLQ FWTKTLPQKI QELKGSQERH KELLEHHHHH H
- 纯度
- > 95 % by SDS - PAGE
- 内毒素水平
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Biological Activity Comment
- Specific activity is > 30 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 umole of p-nitrophenyl acetate to p-nitrophenol per minute at pH 7.5 at 25C.
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- Discover our top product CES2E 蛋白
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Carboxylesterase 2E (CES2E) protein (His tag)
CES2E 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Carboxylesterase 2E (CES2E) (Active) protein (His tag)CES2E 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 88 % as determined by SDS-PAGE Active
Carboxylesterase 2E (CES2E) (AA 27-559) protein (His tag)Crystallography grade CES2E 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Bioactivity Validated
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.25 mg/mL
- 缓冲液
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- 抗原
- Carboxylesterase 2E (CES2E)
- 别名
- Pyrethroid hydrolase Ces2e (CES2E 产品)
- 别名
- 9030624L02Rik Protein, Ces5 Protein, carboxylesterase 2E Protein, Ces2e Protein
- 背景
- CES2E, also known as pyrethroid hydrolase Ces2e, is a type of enzyme that capable of hydrolyzing a variety of carboxylic acid esters and it is widely distributed in cells especially in mammalian liver. It is involved in the chemical reaction, exerting its role in catalyzing the carboxylic ester and water to convert to an alcohol and a carboxylate. Recombinant mouse CES2E, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- 分子量
- 60.5kDa (541aa) 50-70kDa (SDS-PAGE under reducing conditions)
- NCBI登录号
- NP_766347
- UniProt
- Q8BK48
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