GOT1 Protein (AA 1-413) (His tag)
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- 抗原 See all GOT1 蛋白
- GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 1-413
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宿主
- 小鼠
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资源
- 大肠杆菌（E. Coli）
- 标记
- This GOT1 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MGSSHHHHHH SSGLVPRGSH MGSMAPPSVF AQVPQAPPVL VFKLTADFRD DPDPRKVNLG VGAYRTDESQ PWVLPVVRKV EQKIANDNSL NHEYLPILGL AEFRSCASRL VLGDNSPAIR ENRVGGVQSL GGTGALRIGA DFLGRWYNGT DNKNTPIYVS SPTWENHNAV FSAAGFKDIR PYCYWDAEKR GLDLQGFLND LENAPEFSIF VLHACAHNPT GTDPTPEQWK QIAAVMQRRF LFPFFDSAYQ GFASGDLEKD AWAIRYFVSE GFELFCAQSF SKNFGLYNER VGNLTVVGKE SDSVLRVLSQ MEKIVRITWS NPPAQGARIV AATLSDPELF KEWKGNVKTM ADRILTMRSE LRARLEALKT PGTWSHITEQ IGMFSFTGLN PKQVEYLVNE KHIYLLPSGR INMCGLTTKN LDYVATSIHE AVTKIQ
- 纯度
- > 95 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is > 80 units/mg, and is defined as the amount of enzyme that converts 1.0 micromole of alpha-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25C.
- Top Product
- Discover our top product GOT1 蛋白
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Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) (AA 2-413) protein (His tag)
GOT1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) (AA 1-413) protein (GST tag)GOT1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) (AA 2-413) protein (His tag)Crystallography grade GOT1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) (AA 2-413) protein (His tag)GOT1 宿主: 人 宿主: Yeast (Pichia pastoris) Recombinant > 95 % SDS, WB, ELISA, IP
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Bioactivity Validated
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Liquid, In Phosphate buffered saline ( pH 7.4) containing 10 % glycerol, 1 mM DTT
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- 抗原
- GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))
- 别名
- Got1 (GOT1 产品)
- 别名
- ASTQTL1 Protein, GIG18 Protein, cAspAT Protein, cCAT Protein, AST Protein, ISSD Protein, NSD Protein, SD Protein, SIALIN Protein, SIASD Protein, SLD Protein, xr406 Protein, CASPAT Protein, ASPARTATE AMINOTRANSFERASE Protein, ATAAT Protein, MATERNAL EFFECT EMBRYO ARREST 17 Protein, MEE17 Protein, T26C19.9 Protein, T26C19_9 Protein, aspartate aminotransferase Protein, aatA Protein, 83.t00021 Protein, AI789014 Protein, Got-1 Protein, Aspat Protein, Gaspat Protein, AL022787 Protein, FABP-pm Protein, Got-2 Protein, mAspAT Protein, ASPATA Protein, glutamic-oxaloacetic transaminase 1 Protein, solute carrier family 17 member 5 Protein, glutamic-oxaloacetic transaminase 1 homeolog Protein, aspartate aminotransferase Protein, glutamic-oxaloacetic transaminase 1, soluble Protein, glutamic-oxaloacetic transaminase 2 Protein, glutamatic-oxaloacetic transaminase 2, mitochondrial Protein, GOT1 Protein, SLC17A5 Protein, got1.S Protein, AAT Protein, aspC Protein, EHI_006080 Protein, AST2 Protein, ast2 Protein, got1 Protein, Got1 Protein, GOT2 Protein, Got2 Protein
- 背景
- Got1 also known as Glutamate oxaloacetate transaminase 1 is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT1 plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Recombinant mouse Got1 fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- 分子量
- 48.6kDa (436aa) confirmed by MALDI-TOF
- 途径
- Hepatitis C, Monocarboxylic Acid Catabolic Process, Methionine Biosynthetic Process
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