RPRD1B Protein (AA 1-326) (His tag)
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- 抗原 See all RPRD1B 蛋白
- RPRD1B (Regulation of Nuclear Pre-mRNA Domain Containing 1B (RPRD1B))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-326
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This RPRD1B protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MGSSHHHHHH SSGLVPRGSH MGSMSSFSES ALEKKLSELS NSQQSVQTLS LWLIHHRKHA GPIVSVWHRE LRKAKSNRKL TFLYLANDVI QNSKRKGPEF TREFESVLVD AFSHVAREAD EGCKKPLERL LNIWQERSVY GGEFIQQLKL SMEDSKSPPP KATEEKKSLK RTFQQIQEEE DDDYPGSYSP QDPSAGPLLT EELIKALQDL ENAASGDATV RQKIASLPQE VQDVSLLEKI TDKEAAERLS KTVDEACLLL AEYNGRLAAE LEDRRQLARM LVEYTQNQKD VLSEKEKKLE EYKQKLARVT QVRKELKSHI QSLPDLSLLP NVTGGLAPLP SAGDLFSTD
- 纯度
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product RPRD1B 蛋白
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Regulation of Nuclear Pre-mRNA Domain Containing 1B (RPRD1B) (AA 1-326) protein (His tag)
RPRD1B 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Regulation of Nuclear Pre-mRNA Domain Containing 1B (RPRD1B) (AA 1-326) protein (GST tag)RPRD1B 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Regulation of Nuclear Pre-mRNA Domain Containing 1B (RPRD1B) protein (Myc-DYKDDDDK Tag)RPRD1B 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Liquid, In Phosphate buffered saline ( pH 7.4) containing 20 % glycerol, 1 mM DTT
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- 抗原
- RPRD1B (Regulation of Nuclear Pre-mRNA Domain Containing 1B (RPRD1B))
- 别名
- RPRD1B (RPRD1B 产品)
- 别名
- fb23h08 Protein, zgc:55687 Protein, wu:fb23h08 Protein, rprd1ba Protein, MGC130705 Protein, C13H20ORF77 Protein, C20orf77 Protein, CREPT Protein, NET60 Protein, dJ1057B20.2 Protein, 2610304G08Rik Protein, 2810446G03Rik Protein, Crept Protein, RGD1304782 Protein, regulation of nuclear pre-mRNA domain containing 1B Protein, regulation of nuclear pre-mRNA domain containing 1B L homeolog Protein, rprd1b Protein, rprd1b.L Protein, RPRD1B Protein, Rprd1b Protein
- 背景
- RPRD1B also known as regulation of nuclear pre-mRNA domain-containing protein 1B. This protein interacts with phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD. It promotes binding of RNA polymerase II to the CCDN1 promoter and to the termination region before the poly-A site but decreases its binding after the poly-A site. It prevents RNA polymerase II from reading through the 3' end termination site and may allow it to be recruited back to the promoter through promotion of the formation of a chromatin loop. It also enhances the transcription of a number of other cell cycle-related genes including CDK2, CDK4, CDK6 and cyclin-E but not CDKN1A, CDKN1B or cyclin-A. It promotes cell proliferation. Recombinant human RPRD1B, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- 分子量
- 39.3kDa (349aa) confirmed by MALDI-TOF
- NCBI登录号
- NP_067038
- UniProt
- Q9NQG5
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