RNGTT Protein (AA 1-597) (His tag)
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- 抗原 See all RNGTT 蛋白
- RNGTT (RNA Guanylyltransferase and 5'-Phosphatase (RNGTT))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-597
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This RNGTT protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MGSSHHHHHH SSGLVPRGSH MGSMAHNKIP PRWLNCPRRG QPVAGRFLPL KTMLGPRYDS QVAEENRFHP SMLSNYLKSL KVKMGLLVDL TNTSRFYDRN DIEKEGIKYI KLQCKGHGEC PTTENTETFI RLCERFNERN PPELIGVHCT HGFNRTGFLI CAFLVEKMDW SIEAAVATFA QARPPGIYKG DYLKELFRRY GDIEEAPPPP LLPDWCFEDD EDEDEDEDGK KESEPGSSAS FGKRRKERLK LGAIFLEGVT VKGVTQVTTQ PKLGEVQQKC HQFCGWEGSG FPGAQPVSMD KQNIKLLDLK PYKVSWKADG TRYMMLIDGT NEVFMIDRDN SVFHVSNLEF PFRKDLRMHL SNTLLDGEMI IDRVNGQAVP RYLIYDIIKF NSQPVGDCDF NVRLQCIERE IISPRHEKMK TGLIDKTQEP FSVRNKPFFD ICTSRKLLEG NFAKEVSHEM DGLIFQPTGK YKPGRCDDIL KWKPPSLNSV DFRLKITRMG GEGLLPQNVG LLYVGGYERP FAQIKVTKEL KQYDNKIIEC KFENNSWVFM RQRTDKSFPN AYNTAMAVCN SISNPVTKEM LFEFIDRCTA ASQGQKRKHH LDPDTELMPP PPPKRPRPLT
- 纯度
- > 85 % by SDS - PAGE
- Top Product
- Discover our top product RNGTT 蛋白
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RNA Guanylyltransferase and 5'-Phosphatase (RNGTT) (AA 1-597) protein (GST tag)
RNGTT 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
RNA Guanylyltransferase and 5'-Phosphatase (RNGTT) (AA 1-597) protein (Strep Tag)Crystallography grade RNGTT 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
RNA Guanylyltransferase and 5'-Phosphatase (RNGTT) (AA 1-597) protein (Strep Tag)Crystallography grade RNGTT 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
RNA Guanylyltransferase and 5'-Phosphatase (RNGTT) protein (Myc-DYKDDDDK Tag)RNGTT 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.25 mg/mL
- 缓冲液
- Liquid. In 20 mM Tris-HCl buffer ( pH 7.5) containing 0.2M NaCl, 40 % glycerol, 2 mM DTT, 0.1 mM PMSF
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- 抗原
- RNGTT (RNA Guanylyltransferase and 5'-Phosphatase (RNGTT))
- 别名
- RNGTT (RNGTT 产品)
- 别名
- xcap1b Protein, MGC75787 Protein, CAP1A Protein, HCE Protein, HCE1 Protein, hCAP Protein, AU020997 Protein, zgc:76886 Protein, RNA guanylyltransferase and 5'-phosphatase Protein, mRNA-capping enzyme Protein, rngtt.S Protein, rngtt Protein, RNGTT Protein, LOC100439097 Protein, Rngtt Protein
- 背景
- RNGTT is a bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. It catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus. Recombinant human RNGTT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- 分子量
- 70.9 kDa (620aa)
- NCBI登录号
- NP_003791
- UniProt
- O60942
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