ZWILCH Protein (AA 1-591) (His tag)
-
- 抗原 See all ZWILCH 蛋白
- ZWILCH (Zwilch, Kinetochore Associated, Homolog (ZWILCH))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-591
- 宿主
- 人
-
资源
- 大肠杆菌（E. Coli）
- 标记
- This ZWILCH protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MGSSHHHHHH SSGLVPRGSH MGSMWERLNC AAEDFYSRLL QKFNEEKKGI RKDPFLYEAD VQVQLISKGQ PNPLKNILNE NDIVFIVEKV PLEKEETSHI EELQSEETAI SDFSTGENVG PLALPVGKAR QLIGLYTMAH NPNMTHLKIN LPVTALPPLW VRCDSSDPEG TCWLGAELIT TNNSITGIVL YVVSCKADKN YSVNLENLKN LHKKRHHLST VTSKGFAQYE LFKSSALDDT ITASQTAIAL DISWSPVDEI LQIPPLSSTA TLNIKVESGE PRGPLNHLYR ELKFLLVLAD GLRTGVTEWL EPLEAKSAVE LVQEFLNDLN KLDGFGDSTK KDTEVETLKH DTAAVDRSVK RLFKVRSDLD FAEQLWCKMS SSVISYQDLV KCFTLIIQSL QRGDIQPWLH SGSNSLLSKL IHQSYHGTMD TVSLSGTIPV QMLLEIGLDK LKKDYISFFI GQELASLNHL EYFIAPSVDI QEQVYRVQKL HHILEILVSC MPFIKSQHEL LFSLTQICIK YYKQNPLDEQ HIFQLPVRPT AVKNLYQSEK PQKWRVEIYS GQKKIKTVWQ LSDSSPIDHL NFHKPDFSEL TLNGSLEERI FFTNMVTCSQ VHFK
- 纯度
- > 85 % by SDS - PAGE
- Top Product
- Discover our top product ZWILCH 蛋白
-
Zwilch, Kinetochore Associated, Homolog (ZWILCH) (AA 1-477) protein (GST tag)
ZWILCH 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Zwilch, Kinetochore Associated, Homolog (ZWILCH) (AA 1-591) protein (Strep Tag)Crystallography grade ZWILCH 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 0.25 mg/mL
- 缓冲液
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.1M NaCl, 20 % glycerol, 1 mM DTT
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
-
- 抗原
- ZWILCH (Zwilch, Kinetochore Associated, Homolog (ZWILCH))
- 别名
- ZWILCH (ZWILCH 产品)
- 别名
- CG18639 Protein, CG18729 Protein, Dmel\\CG18729 Protein, Zwilch Protein, hzwilch Protein, kntc1ap Protein, KNTC1AP Protein, hZwilch Protein, 2310031L18Rik Protein, si:ch211-242m18.4 Protein, wu:fb10f09 Protein, zwilch kinetochore protein Protein, CG18729 gene product from transcript CG18729-RA Protein, protein zwilch homolog Protein, Zwilch Protein, zwilch Protein, LOC100374963 Protein, ZWILCH Protein
- 背景
- ZWILCH belongs to the ZWILCH family. This protein is an essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. It is required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Recombinant human ZWILCH protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- 分子量
- 69.6kDa(614aa)
- NCBI登录号
- NP_060445
- UniProt
- Q9H900
-
