SHFM1, as known as 26S proteasome complex subunit DSS1, has been proposed to be a candidate gene for the autosomal dominant form of the heterogeneous limb developmental disorder split hand/split foot malformation type 1. In addition, it has been shown to directly interact with BRCA2. It also may play a role in the completion of the cell cycle. Recombinant human SHFM1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
分子量
10.7kDa (93aa) confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)