DBR1 Protein (AA 228-544) (His tag)
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- 抗原 See all DBR1 蛋白
- DBR1 (Debranching Enzyme Homolog 1 (DBR1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 228-544
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This DBR1 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MGSSHHHHHH SSGLVPRGSH MGSHVKFAAL MQHQAKDKGQ TARATKFLAL DKCLPHRDFL QILEIEHDPS APDYLEYDIE WLTILRATDD LINVTGRLWN MPENNGLHAR WDYSATEEGM KEVLEKLNHD LKVPCNFSVT AACYDPSKPQ TQMQLIHRIN PQTTEFCAQL GIIDINVRLQ KSKEEHHVCG EYEEQDDVES NDSGEDQSEY NTDTSALSSI NPDEIMLDEE EDEDSIVSAH SGMNTPSVEP SDQASEFSAS FSDVRILPGS MIVSSDDTVD STIDREGKPG GTVESGNGED LTKVPLKRLS DEHEPEQRKK IKRRNQAIYA AVDDDDDDAA
- 纯度
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product DBR1 蛋白
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Debranching Enzyme Homolog 1 (DBR1) (AA 1-544) protein (GST tag)
DBR1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Debranching Enzyme Homolog 1 (DBR1) (AA 445-538) protein (GST tag)DBR1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Debranching Enzyme Homolog 1 (DBR1) (AA 1-544) protein (Strep Tag)Crystallography grade DBR1 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Debranching Enzyme Homolog 1 (DBR1) (AA 1-550) protein (Strep Tag)Crystallography grade DBR1 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Debranching Enzyme Homolog 1 (DBR1) protein (Myc-DYKDDDDK Tag)DBR1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 20 % glycerol, 1 mM DTT.
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- 抗原
- DBR1 (Debranching Enzyme Homolog 1 (DBR1))
- 别名
- DBR1 (DBR1 产品)
- 别名
- AW018415 Protein, wu:fc08f08 Protein, zgc:63930 Protein, ARABIDOPSIS THALIANA DEBRANCHING ENZYME 1 Protein, ATDBR1 Protein, F28M20.40 Protein, F28M20_40 Protein, debranching enzyme 1 Protein, dbr1 Protein, dbr1-a Protein, dbr1-b Protein, debranching RNA lariats 1 Protein, debranching enzyme homolog 1 (S. cerevisiae) Protein, Lariat debranching enzyme Protein, debranching enzyme 1 Protein, RNA lariat debranching enzyme Dbr1 Protein, debranching RNA lariats 1 S homeolog Protein, debranching RNA lariats 1 L homeolog Protein, DBR1 Protein, Dbr1 Protein, dbr1 Protein, dbr-1 Protein, dbr1.S Protein, dbr1.L Protein
- 背景
- DBR1 is an RNA lariat debranching enzyme that hydrolyzes 2'-5' prime branched phosphodiester bonds. The encoded protein specifically targets the bonds at the branch point of excised lariat intron RNA, converting them to linear molecules that are then degraded. This protein may also be involved in retroviral replication. Recombinant human DBR1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- 分子量
- 38 kDa (340aa) confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)
- NCBI登录号
- NP_057300
- UniProt
- Q9UK59
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