PPM1F Protein (AA 1-454) (His tag)
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- 抗原 See all PPM1F 蛋白
- PPM1F (Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-454
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This PPM1F protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MGSSHHHHHH SSGLVPRGSH MGSMSSGAPQ KSSPMASGAE ETPGFLDTLL QDFPALLNPE DPLPWKAPGT VLSQEEVEGE LAELAMGFLG SRKAPPPLAA ALAHEAVSQL LQTDLSEFRK LPREEEEEEE DDDEEEKAPV TLLDAQSLAQ SFFNRLWEVA GQWQKQVPLA ARASQRQWLV SIHAIRNTRR KMEDRHVSLP SFNQLFGLSD PVNRAYFAVF DGHGGVDAAR YAAVHVHTNA ARQPELPTDP EGALREAFRR TDQMFLRKAK RERLQSGTTG VCALIAGATL HVAWLGDSQV ILVQQGQVVK LMEPHRPERQ DEKARIEALG GFVSHMDCWR VNGTLAVSRA IGDVFQKPYV SGEADAASRA LTGSEDYLLL ACDGFFDVVP HQEVVGLVQS HLTRQQGSGL RVAEELVAAA RERGSHDNIT VMVVFLRDPQ ELLEGGNQGE GDPQAEGRRQ DLPSSLPEPE TQAPPRS
- 纯度
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product PPM1F 蛋白
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Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F) (AA 1-454) protein (GST tag)
PPM1F 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F) (AA 1-100) protein (GST tag)PPM1F 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F) (AA 1-454) protein (His tag)PPM1F 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F) protein (Myc-DYKDDDDK Tag)PPM1F 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 20 % glycerol, 1 mM DTT.
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- 抗原
- PPM1F (Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F))
- 别名
- PPM1F (PPM1F 产品)
- 别名
- 1110021B16Rik Protein, 4933427B07Rik Protein, CaMKPase Protein, Popx2 Protein, mKIAA0015 Protein, CAMKP Protein, FEM-2 Protein, POPX2 Protein, hFEM-2 Protein, protein phosphatase, Mg2+/Mn2+ dependent 1F Protein, protein phosphatase 1F (PP2C domain containing) Protein, protein phosphatase, Mg2+/Mn2+ dependent, 1F Protein, PPM1F Protein, Ppm1f Protein
- 背景
- PPM1F is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. Recombinant human PPM1F protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- 分子量
- 52kDa (477aa)
- NCBI登录号
- NP_055449
- UniProt
- P49593
- 途径
- Positive Regulation of Endopeptidase Activity
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