IMPACT Protein (AA 1-320) (His tag)
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- 抗原 See all IMPACT 蛋白
- IMPACT (Impact Homolog (IMPACT))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-320
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This IMPACT protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MGSSHHHHHH SSGLVPRGSH MGSMAEGDAG SDQRQNEEIE AMAAIYGEEW CVIDDCAKIF CIRISDDIDD PKWTLCLQVM LPNEYPGTAP PIYQLNAPWL KGQERADLSN SLEEIYIQNI GESILYLWVE KIRDVLIQKS QMTEPGPDVK KKTEEEDVEC EDDLILACQP ESSVKALDFD ISETRTEVEV EELPPIDHGI PITDRRSTFQ AHLAPVVCPK QVKMVLSKLY ENKKIASATH NIYAYRIYCE DKQTFLQDCE DDGETAAGGR LLHLMEILNV KNVMVVVSRW YGGILLGPDR FKHINNCARN ILVEKNYTNS PEESSKALGK NKKVRKDKKR NEH
- 纯度
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product IMPACT 蛋白
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Impact Homolog (IMPACT) (AA 1-320) protein (His tag)
IMPACT 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Impact Homolog (IMPACT) (AA 1-320) protein (GST tag)IMPACT 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Impact Homolog (IMPACT) (AA 1-320) protein (Strep Tag)Crystallography grade IMPACT 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Impact Homolog (IMPACT) (AA 1-318) protein (Strep Tag)Crystallography grade IMPACT 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Impact Homolog (IMPACT) protein (Myc-DYKDDDDK Tag)IMPACT 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 10 % glycerol, 1 mM DTT
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- 抗原
- IMPACT (Impact Homolog (IMPACT))
- 别名
- IMPACT (IMPACT 产品)
- 别名
- zgc:103672 Protein, Ximpact Protein, impact-A Protein, RWDD5 Protein, impact-a Protein, E430016J11Rik Protein, impact RWD domain protein Protein, impact Protein, impact RWD domain protein L homeolog Protein, impact, RWD domain protein Protein, IMPACT Protein, impact Protein, EDI_298310 Protein, impact.L Protein, Impact Protein
- 背景
- IMPACT belongs to the IMPACT family and contains 1 RWD domain. This protein is translational regulator that ensures constant high levels of translation under amino acid starvation and acts by interacting with GCN1/GCN1L1, thereby preventing activation of GCN2 protein kinases (EIF2AK1 to 4) and subsequent down-regulation of protein synthesis. Recombinant human IMPACT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- 分子量
- 38.9kDa (343aa), confirmed by MALDI-TOF
- NCBI登录号
- NP_060909
- UniProt
- Q9P2X3
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