OTUB1 Protein (AA 2-201, partial) (GST tag)
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- 抗原 See all OTUB1 蛋白
- OTUB1 (OTU Domain, Ubiquitin Aldehyde Binding 1 (OTUB1))
- 蛋白类型
- Recombinant
- 产品特性
- partial, AA 2-201
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This OTUB1 protein is labelled with GST tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- AAEEPQQQKQ EPLGSDSEGV NCLAYDEAIM AQQDRIQQEI AVQNPLVSER LELSVLYKEY AEDDNIYQQK IKDLHKKYSY IRKTRPDGNC FYRAFGFSHL EALLDDSKEL QRFKAVSAKS KEDLVSQGFT EFTIEDFHNT FMDLIEQVEK QTSVADLLAS FNDQSTSDYL VVYLRLLTSG YLQRESKFFE HFIEGGRTVK
- 纯化方法
- SDS-PAGE
- 纯度
- > 90 %
- Top Product
- Discover our top product OTUB1 蛋白
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OTU Domain, Ubiquitin Aldehyde Binding 1 (OTUB1) (AA 1-271) protein (GST tag)
OTUB1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
OTU Domain, Ubiquitin Aldehyde Binding 1 (OTUB1) (AA 2-201), (partial) protein (GST tag)OTUB1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 95 % SDS, WB, ELISA
OTU Domain, Ubiquitin Aldehyde Binding 1 (OTUB1) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)OTUB1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.1-2 mg/mL
- 缓冲液
- 20 mM Tris-HCl based buffer, pH 8.0
- 储存条件
- -80 °C,4 °C,-20 °C
- 储存方法
- Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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- 抗原
- OTUB1 (OTU Domain, Ubiquitin Aldehyde Binding 1 (OTUB1))
- 别名
- OTUB1 (OTUB1 产品)
- 别名
- OTB1 Protein, OTU1 Protein, AI850305 Protein, fa19h07 Protein, otub1 Protein, wu:fa19h07 Protein, zgc:92839 Protein, MGC131231 Protein, fb17f04 Protein, otub1l Protein, wu:fb17f04 Protein, zgc:92685 Protein, OTU deubiquitinase, ubiquitin aldehyde binding 1 Protein, OTU domain, ubiquitin aldehyde binding 1 Protein, OTU deubiquitinase, ubiquitin aldehyde binding 1b Protein, OTU deubiquitinase, ubiquitin aldehyde binding 1 L homeolog Protein, similar to HSPC263 Protein, OTU deubiquitinase, ubiquitin aldehyde binding 1a Protein, OTUB1 Protein, Otub1 Protein, otub1b Protein, otub1.L Protein, otub1 Protein, RGD1565010 Protein, otub1a Protein
- 背景
- Hydrolase that can specifically rove 'Lys-48'-linked conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation. Regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. Acts via its interaction with RNF128/GRAIL, a crucial inductor of CD4 T-cell anergy. Isoform 1 destabilizes RNF128, leading to prevent anergy. In contrast, isoform 2 stabilizes RNF128 and promotes anergy. Surprisingly, it regulates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128. Deubiquitinates estrogen receptor alpha (ESR1). Mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains, but not 'Lys-63'-linked polyubiquitin chains. Not able to cleave di-ubiquitin. Also capable of roving NEDD8 from NEDD8 conjugates, but with a much lower preference compared to 'Lys-48'-linked ubiquitin.Plays a key non-catalytic role in DNA repair regulation by inhibiting activity of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of 'Lys-63'-linked histone H2A and H2AX at DNA damage sites. Inhibits RNF168 independently of ubiquitin thioesterase activity by binding and inhibiting UBE2N/UBC13, the E2 partner of RNF168, thereby limiting spreading of 'Lys-63'-linked histone H2A and H2AX marks. Inhibition occurs by binding to free ubiquitin: free ubiquitin acts as an allosteric regulator that increases affinity for UBE2N/UBC13 and disrupts interaction with UBE2V1. The OTUB1-UBE2N/UBC13-free ubiquitin complex adopts a configuration that mimics a cleaved 'Lys48'-linked di-ubiquitin chain.
- 分子量
- 50.5 kDa
- UniProt
- Q96FW1
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