POLN Protein (AA 60-240) (His tag)
POLN
宿主: Hepatitis E Virus (HEV)
宿主: 大肠杆菌（E. Coli）
Recombinant
> 90 %
SDS
1 image
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- 抗原 See all POLN products
- POLN (Polymerase (DNA Directed) nu (POLN))
- 蛋白类型
- Recombinant
- 产品特性
- AA 60-240
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宿主
- Hepatitis E Virus (HEV)
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资源
- 大肠杆菌（E. Coli）
- 标记
- This POLN protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- EVFWNHPIQR VIHNELELYC RARSGRCLEI GAHPRSINDN PNVVHRCFLR PAGRDVQRWY TAPTRGPAAN CRRSALRGLP AADRTYCFDG FSGCNFPAET GIALYSLHDM SPSDVAEAMF RHGMTRLYAA LHLPPEVLLP PGTYRTASYL LIHDGRRVVV TYEGDTSAGY NHDVSNLRSW I
- 纯化方法
- SDS-PAGE
- 纯度
- > 90 %
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Polymerase (DNA Directed) nu (POLN) (AA 1-100) protein (GST tag)
POLN 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.1-2 mg/mL
- 缓冲液
- 20 mM Tris-HCl based buffer, pH 8.0
- 储存条件
- -80 °C,4 °C,-20 °C
- 储存方法
- Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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- 抗原
- POLN (Polymerase (DNA Directed) nu (POLN))
- 别名
- POLN (POLN 产品)
- 别名
- POLN Protein, POL4P Protein, DNA polymerase nu Protein, polymerase (DNA directed) nu Protein, DNA polymerase N Protein, POLN Protein, Poln Protein
- 物质类
- Viral Protein
- 背景
- Methyltransferase displays a cytoplasmic capping enzyme activity. This function is necessary since all viral RNAs are synthesized in the cytoplasm, and host capping enzymes are restricted to the nucleus. The enzymatic reaction involves a covalent link between 7-methyl-GMP and the methyltransferase, whereas eukaryotic capping enzymes form a covalent complex only with GMP. Methyltransferase catalyzes transfer of a methyl group from S-adenosylmethionine to GTP and GDP to yield m7GTP or m7GDP. GMP, GpppG, and GpppA were poor substrates for the methyltransferase. This enzyme also displays guanylyltransferase activity to form a covalent complex, methyltransferase-m7GMP, from which 7-methyl-GMP is transferred to the mRNA to create the cap structure. Cap analogs such as m7GTP, m7GDP, et2m7GMP, and m2et7GMP inhibit the methyltransferase reaction .RNA-directed RNA polymerase plays an essential role in the virus replication. Binds to the 3'-end of the genomic RNA, probably to initiate replication ..
- 分子量
- 24.5 kDa
- UniProt
- P33424
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