UBE2D3 Protein (AA 1-147, full length) (His-SUMO Tag)
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- 抗原 See all UBE2D3 蛋白
- UBE2D3 (Ubiquitin-Conjugating Enzyme E2D 3 (UBE2D3))
- 蛋白类型
- Recombinant
- 产品特性
- full length, AA 1-147
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This UBE2D3 protein is labelled with His-SUMO Tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MALKRINKEL SDLARDPPAQ CSAGPVGDDM FHWQATIMGP NDSPYQGGVF FLTIHFPTDY PFKPPKVAFT TRIYHPNINS NGSICLDILR SQWSPALTIS KVLLSICSLL CDPNPDDPLV PEIARIYKTD RDKYNRISRE WTQKYAM
- 纯化方法
- SDS-PAGE
- 纯度
- > 90 %
- Top Product
- Discover our top product UBE2D3 蛋白
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Ubiquitin-Conjugating Enzyme E2D 3 (UBE2D3) (AA 1-149) protein (His tag)
UBE2D3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % by SDS - PAGE SDS
Ubiquitin-Conjugating Enzyme E2D 3 (UBE2D3) (AA 1-147) protein (His tag)UBE2D3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Ubiquitin-Conjugating Enzyme E2D 3 (UBE2D3) (AA 1-147) protein (GST tag)UBE2D3 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Ubiquitin-Conjugating Enzyme E2D 3 (UBE2D3) (AA 1-147) protein (Strep Tag)Crystallography grade UBE2D3 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
Ubiquitin-Conjugating Enzyme E2D 3 (UBE2D3) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)UBE2D3 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Ubiquitin-Conjugating Enzyme E2D 3 (UBE2D3) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)UBE2D3 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Ubiquitin-Conjugating Enzyme E2D 3 (UBE2D3) (Transcript Variant 5) protein (Myc-DYKDDDDK Tag)UBE2D3 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.1-2 mg/mL
- 缓冲液
- 20 mM Tris-HCl based buffer, pH 8.0
- 储存条件
- -80 °C,4 °C,-20 °C
- 储存方法
- Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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- 抗原
- UBE2D3 (Ubiquitin-Conjugating Enzyme E2D 3 (UBE2D3))
- 别名
- UB2D3 (UBE2D3 产品)
- 别名
- E2(17)KB3 Protein, UBC4/5 Protein, UBCH5C Protein, eff Protein, fb36f02 Protein, wu:fb36f02 Protein, zgc:56340 Protein, 1100001F19Rik Protein, 9430029A22Rik Protein, AA414951 Protein, UBE2D3P Protein, Xubc4 Protein, e2(17)kb3 Protein, ubc4/5 Protein, ubch5c Protein, ube2d3.1 Protein, ube2d2 Protein, xubc4 Protein, wu:fj13d01 Protein, zgc:73200 Protein, ubiquitin conjugating enzyme E2 D3 Protein, ubiquitin-conjugating enzyme E2D 3 Protein, ubiquitin conjugating enzyme E2 D3 L homeolog Protein, ubiquitin-conjugating enzyme E2D 2 (UBC4/5 homolog, yeast), like Protein, UBE2D3 Protein, ube2d3 Protein, Ube2d3 Protein, LOC100223566 Protein, ube2d3.L Protein, ube2d2l Protein
- 背景
- Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'-linked polyubiquitination. Cooperates with the E2 CDC34 and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Ubiquitin chain elongation is then performed by CDC34, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. Acts also as an initiator E2, in conjunction with RNF8, for the priming of PCNA. Monoubiquitination of PCNA, and its subsequent polyubiquitination, are essential events in the operation of the DNA damage tolerance (DDT) pathway that is activated after DNA damage caused by UV or chical agents during S-phase. Associates with the BRCA1/BARD1 E3 ligase complex to perform ubiquitination at DNA damage sites following ionizing radiation leading to DNA repair. Targets DAPK3 for ubiquitination which influences promyelocytic leukia protein nuclear body (PML-NB) formation in the nucleus. In conjunction with the MDM2 and TOPORS E3 ligases, functions ubiquitination of p53/TP53. Supports NRDP1-mediated ubiquitination and degradation of ERBB3 and of BRUCE which triggers apoptosis. In conjunction with the CBL E3 ligase, targets EGFR for polyubiquitination at the plasma mbrane as well as during its internalization and transport on endosomes. In conjunction with the STUB1 E3 quality control E3 ligase, ubiquitinates unfolded proteins to catalyze their immediate destruction .
- 分子量
- 32.7 kDa
- UniProt
- P61077
- 途径
- Activation of Innate immune Response, Toll-Like Receptors Cascades
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