CXCL12 Protein (AA 23-89) (His tag,GST tag)
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- 抗原 See all CXCL12 蛋白
- CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))
- 生物活性
- Active
- 产品特性
- AA 23-89
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This CXCL12 protein is labelled with His tag,GST tag.
- 应用范围
- Cell Culture (CC), Activity Assay (AcA)
- 产品特性
- Tag location: N-terminal His and GST Tag
- 纯度
- > 97 %
- Biological Activity Comment
- SDF1 (stromal cell-derived factor 1), also known as C-X-C motif chemokine 12, is a chemokine protein that has chemotaxis active on T-lymphocytes and monocytes. It is thought that SDF1 stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3; thus, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of SDF1 on the human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into the upper chambers (100uL cell suspension,106cells/mL in RPMI 1640 with 0.5% FBS) and SDF1 (10ng/mL, 30ng/mL and 60ng/mL diluted separately in serum free RPMI 1640) was added in lower chamber with a polycarbonate filter (8μm pore size) used to separate the two compartments. After incubation at 37°C with 5% CO2 for 3h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (x100) and the number of migrated cells were counted at high magnification (x400) randomly (five fields for each filter). Result shows SDF1 is able to induce migration of THP-1 cells. The migrated THP-1 cells in low chamber at low magnification (x100) were shown in Figure 1. Five fields of each chamber were randomly chosen, and the migrated cells were counted at high magnification (x400). Statistical results were shown in Figure 2. The optimum chemotaxis of SDF1 occurs at 10ng/mL.
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Chemokine (C-X-C Motif) Ligand 12 (CXCL12) (AA 23-89) (Active) protein (His tag,GST tag)
CXCL12 宿主: 人 宿主: 大肠杆菌（E. Coli） > 97 % CC, AcA Active
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) (AA 22-89) protein (His tag)CXCL12 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) (AA 22-89) protein (His tag)CXCL12 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) (AA 1-89) protein (GST tag)CXCL12 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) (AA 24-89) (Active) protein (Biotin)CXCL12 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 97 % by SDS PAGE FACS, CMA Active
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) (AA 22-93) protein (His tag)Crystallography grade CXCL12 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) (AA 24-88) protein (His tag)Crystallography grade CXCL12 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) (AA 24-93) protein (His tag)Crystallography grade CXCL12 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) (AA 22-93) protein (His tag)Crystallography grade CXCL12 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 应用备注
- Isoelectric Point: 8.5
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))
- 别名
- Stromal Cell Derived Factor 1 (SDF1) (CXCL12 产品)
- 别名
- IRH Protein, PBSF Protein, SCYB12 Protein, SDF1 Protein, TLSF Protein, TPAR1 Protein, CINC-1 Protein, Gro1 Protein, Pbsf Protein, Scyb12 Protein, Sdf1 Protein, Tlsf Protein, Tpar1 Protein, Fsp Protein, KC Protein, Mgsa Protein, N51 Protein, Scyb1 Protein, gro Protein, sdf-1 Protein, sdf1 Protein, xSDF-1 Protein, SDF-1 Protein, cxcl12 Protein, sdf-1a Protein, sdf1a Protein, wu:fa55e10 Protein, wu:fc16h12 Protein, wu:fj84c02 Protein, C-X-C motif chemokine ligand 12 Protein, C-X-C motif chemokine ligand 1 Protein, chemokine (C-X-C motif) ligand 12 Protein, chemokine (C-X-C motif) ligand 1 Protein, C-X-C motif chemokine ligand 12 L homeolog Protein, chemokine (C-X-C motif) ligand 12a (stromal cell-derived factor 1) Protein, CXCL12 Protein, cxcl12 Protein, Cxcl1 Protein, Cxcl12 Protein, cxcl12.L Protein, cxcl12a Protein
- 背景
- Alternative Names: CXCL12, PBSF, P-BSF, SCYB12, SDF1-A, SDF1-B, SDF1A, SDF1B, TLSF-A, TLSF-B, TPAR1, Pre-B Cell Growth-Stimulating Factor, Chemokine C-X-C-Motif Ligand 12
- 分子量
- 40kDa
- UniProt
- P48061
- 途径
- Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling
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