Quick Overview for MEK1 蛋白 (ABIN5624610)
抗原
See all MEK1 (MAP2K1) 蛋白蛋白类型
宿主
资源
应用范围
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Supplier Product No.
- 009-001-gq3
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Supplier
- Rockland
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原理
- MEK1 human recombinant protein
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产品特性
- MAP2K, MEK, MEK1, MKK1, PRKMK1, MEK-1 recombinant protein
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纯化方法
- MEK1 is a recombinant protein containing a polyhistidine tag expressed in Sf9. Analysis by SDS-PAGE resulted in a pattern consistent with purified MEK1 and was estimated to be greater than 90% pure.
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过滤
- Sterile filtered
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Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 1-58) protein (GST tag)
MAP2K1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) protein (GST tag,His tag)MAP2K1 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 95 % as determined by SDS-PAGE
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 294-393) protein (GST tag)MAP2K1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) protein (Myc-DYKDDDDK Tag)MAP2K1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
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Application Note: Human MEK1 recombinant protein has been tested in SDS-Page and is suitable as a control for polyclonal or monoclonal anti-MEK1 in immunological assays. For western blot use at 50 ng or less. For other assays concentration is user optimized. For a phosphorylated form of the protein co-expressed with constitutively active B-raf (V600E) see (p/n 009-001-GP5) MEK1 pS218-pS222.
Western Blot Dilution: 50 ng
Other: Dot Blot
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
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有效期
- 12 months
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- MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))
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别名
- MAP2K1
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背景
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Synonyms: MAP2K, MEK, MEK1, MKK1, PRKMK1, MEK-1 recombinant protein
Background: MEK1 recombinant protein was produced in Sf9 cells. Mitogen-activated protein kinase kinase 1, also known as MKK or MEK, is an integral component of the MAP kinase cascade that regulates cell growth and differentiation. This pathway also plays a key role in synaptic plasticity in the brain. Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase. MEK1 and MEK2 are about 80 % identical to each other, and nearly identical within the kinase domain. Recombinant MEK1 protein is ideal for investigators involved in Neuroscience, Cell Signaling and Cancer Research.
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UniProt
- Q02750
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途径
- MAPK Pathway, RTK signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling
抗原
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