MEK1 Protein (double Mutant)
Quick Overview for MEK1 Protein (double Mutant) (ABIN5624609)
抗原
See all MEK1 (MAP2K1) 蛋白蛋白类型
宿主
资源
应用范围
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产品特性
- double Mutant
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Supplier Product No.
- 009-001-gq6
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Supplier
- Rockland
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原理
- MEK1 double mutant human recombinant protein
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产品特性
- MAP2K, MEK, MEK1, MKK1, PRKMK1, MEK-1 recombinant protein
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纯化方法
- MEK1 double mutant human is a recombinant protein containing a polyhistidine tag expressed in Sf9. This double mutant protein contain alanine substitutions at S218A and S222A rendering it inactive. Analysis by SDS-PAGE resulted in a pattern consistent with purified MEK1 and was estimated to be greater than 90% pure.
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过滤
- Sterile filtered
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Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 1-58) protein (GST tag)
MAP2K1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) protein (GST tag,His tag)MAP2K1 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 95 % as determined by SDS-PAGE
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 294-393) protein (GST tag)MAP2K1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) proteinMAP2K1 宿主: 人 宿主: Insect cells (Sf9) Recombinant WB, SDS
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) protein (Myc-DYKDDDDK Tag)MAP2K1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
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Application Note: Human MEK1 mutant recombinant protein has been tested in SDS-Page and is suitable as a negative control protein for immunoassays using antibodies targeting the critical MEK1 phosphorylated S218 or S222 residues. For western blot use at 50 ng or less. For other assays concentration is user optimized.
Western Blot Dilution: 50 ng
Other: Dot Blot
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
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有效期
- 12 months
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- MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))
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别名
- MAP2K1
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背景
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Synonyms: MAP2K, MEK, MEK1, MKK1, PRKMK1, MEK-1 recombinant protein
Background: MEK1 isoform double mutant recombinant protein is modified to put alanine residues at two key activation sites, Serine 218 and Serine 222. Mitogen-activated protein kinase kinase 1, also known as MKK or MEK, is an integral component of the MAP kinase cascade that regulates cell growth and differentiation. This pathway also plays a key role in synaptic plasticity in the brain. Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase. The inactive double mutant MEK1 recombinant protein is ideal for investigators involved in Neuroscience, Cell Signaling and Cancer Research.
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UniProt
- Q02750
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途径
- MAPK Pathway, RTK signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling
抗原
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