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- 抗原 See all COL3 蛋白
- COL3 (Collagen, Type III (COL3))
- 蛋白类型
- Native
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宿主
- 人
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资源
- Human
- 应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- 纯化方法
- Human Collagen type III has been prepared from human placenta and is chromatographically and immunologically pure. This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. This product reacts with anti-Collagen Type III. Reaction with Rockland's anti-Collagen I, II, IV, V or VI is negligible (typically less than 1% cross reactivity was detected by ELISA).
- 过滤
- Sterile filtered
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- 应用备注
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Immunohistochemistry Dilution: 1:100-1:500
Application Note: Human Collagen type III purified protein standard is used as a control or standard in indirect trapping ELISA for quantitation of antigen using a standard curve, for immunoprecipitation and for other immunological assays as optimized by the user. Specific conditions should be optimized by user. Collagen type III is recognized by type specific anti-collagen antibodies that recognize a native three-dimensional structure.
Western Blot Dilution: 1:1000
ELISA Dilution: 1:10,000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.5 M Acetic Acid
Stabilizer: None
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 6 months
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