Quick Overview for AKT1 蛋白 (ABIN5624567)
抗原
See all AKT1 蛋白蛋白类型
宿主
资源
应用范围
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Supplier Product No.
- 009-001-p21
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Supplier
- Rockland
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原理
- AKT1 Human Recombinant Protein
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纯化方法
- Recombinant protein corresponds to amino acids 1 to 480 of mature human AKT1, Akt isoform 1. The recombinant protein contains a polyhistidine affinity tag at the amino terminus. This protein was co-expressed with the p110 kinase domain leading to phosphorylation of key residues T308 and S473. Purity is greater than 90% as determined by reducing and non-reducing SDS-PAGE and by analytical HPLC.
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!探索我们的预定义定制蛋白和定制蛋白服务！Product表达系统ConjugateOrigin价格从表达系统 HEK-293 CellsConjugate His tagOrigin Human价格从 115,504.49 ¥您的项目需要进一步定制吗？联系我们，了解我们的定制蛋白解决方案
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V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-480) protein (His tag)
custom-made AKT1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-480) protein (His tag)custom-made AKT1 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) proteinAKT1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant WB, SDS, ELISA
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (S473A), (T308A) proteinAKT1 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant WB
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-149) protein (GST tag)AKT1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 381-480) protein (GST tag)AKT1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-480) protein (GST tag)AKT1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-480) protein (His-IF2DI Tag)AKT1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)AKT1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
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Application Note: Human AKT1 recombinant protein has been tested in SDS-Page and western blot and is suitable as a control for polyclonal or monoclonal anti-AKT1 in immunological assays. Akt1 recombinant protein is expected to be phosphorylated and in an active state. It is well suited as a control for anti-AKT pT308 which detects phosphorylated T308 residue, and anti-AKT pS473, that detects phosphorylated S473. For western blot use at 50 ng or less. For other assays concentration is user optimized.
Western Blot Dilution: 50 ng
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.16 mg/mL
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缓冲液
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Buffer: 20 mM Tris pH 8, 300 mM NaCl with 10 % glycerol
Stabilizer: 10 % (v/v) Glycerol
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储存液
- Without preservative
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储存条件
- -20 °C
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储存方法
- Store vial at -20°C or colder prior to use. Avoid cycles of freezing and thawing, aliquot and freeze at -20° C or colder. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature. Thaw only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. For long term storage we recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) .
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有效期
- 12 months
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- AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
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别名
- AKT1 Protein
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背景
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Synonyms: RAC, PKB, AKT, PKB alpha, PRKBA, activated AKT
Background: AKT1 is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and RAC (Related to A and C kinases). AKT is a key regulator of many signal transduction pathways, and it exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation. AKT1 recombinant protein is ideal for investigators involved in Cell Signaling, Neuroscience and Signal Transduction research.
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基因ID
- 207, 62241011
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UniProt
- P31749
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途径
- PI3K-Akt Signaling, RTK signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effect
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