Uchl3
宿主: 人
宿主: 大肠杆菌(E. Coli)
Recombinant
> 95 % as determined by reducing SDS-PAGE.
应用备注
This recombinant protein can be used for WB.
限制
仅限研究用
状态
Lyophilized
缓冲液
50 mM Tris, 150 mM NaCl, pH 7.5
注意事项
Avoid repeated freeze-thaw cycles.
储存条件
-20 °C,-80 °C
储存方法
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C.
Ubiquitin carboxyl-terminal hydrolase isozyme L3 (UCH-L3), a member of peptidase C12 family, is also known as ubiquitin thioesterase L3. Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. UCH-L3 is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. UCH-L3 indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. It is also required for stress-response retinal, skeletal muscle and germ cell maintenance. Furthermore, UCH-L3 may be involved in working memory and can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome and is associated with neurogenerative disorders.