IL-3 蛋白
Quick Overview for IL-3 蛋白 (ABIN5505701)
抗原
See all IL-3 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
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产品特性
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- Recombinant human Interleukin-3 / IL3 protein expressed in Insect cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
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内毒素水平
- Less than 0.01 ng per μg protein as determined by the LAL method.
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Biological Activity Comment
- The ED50 is 0.10 - 0.30 ng/ml, corresponding to a specific activity of 0.33 - 1.0 x 10^7 units/mg, determined by the dose dependent stimulation of TF-1 cell proliferation assay. Under this assay the bioactivity is equivalent to competit
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Interleukin 3 (IL-3) (AA 20-152) protein (His-Avi Tag)
IL-3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 3 (IL-3) (AA 20-143) protein (His tag)IL-3 宿主: Cynomolgus 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 3 (IL-3) (AA 20-152) protein (His-Avi Tag,Biotin)IL-3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 3 (IL-3) protein (His tag)IL-3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
Interleukin 3 (IL-3) (AA 27-166) protein (Fc Tag)IL-3 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 3 (IL-3) (AA 33-166) (Active) proteinIL-3 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE analysis. Active
Interleukin 3 (IL-3) (AA 27-166) protein (His-IF2DI Tag)IL-3 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Interleukin 3 (IL-3) (AA 33-166) (Active) protein (His tag)IL-3 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA Active
Interleukin 3 (IL-3) protein (His tag)IL-3 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Interleukin 3 (IL-3) (AA 1-152) protein (GST tag)IL-3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
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应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays -
限制
- 仅限研究用
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浓度
- > 50 μg/mL
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缓冲液
- 20 mM NaH2PO4, 0.30 M NaCl, pH 6.0
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储存条件
- -80 °C
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储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- IL-3 (Interleukin 3 (IL-3))
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别名
- Interleukin-3 / IL3
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背景
- The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.
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分子量
- 15 kDa
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NCBI登录号
- NP_000579
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途径
- JAK/STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy
抗原
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