Aminoacylase 1 Protein (ACY1) (DYKDDDDK Tag)
Quick Overview for Aminoacylase 1 Protein (ACY1) (DYKDDDDK Tag) (ABIN5505551)
抗原
See all Aminoacylase 1 (ACY1) 蛋白蛋白类型
宿主
资源
应用范围
纯度
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标记
- This Aminoacylase 1 protein is labelled with DYKDDDDK Tag.
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产品特性
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- Recombinant human Purified recombinant protein of Human aminoacylase 1 (ACY1), transcript variant 1, full length, with C-terminal DDK tag, expressed in sf9, 20 μg (full length, C-term DDK tag, transcript variant 1) protein expressed in Sf9 cells.
- Produced with end-sequenced ORF clone
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纯化方法
- Purified
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Aminoacylase 1 (ACY1) (AA 1-408) protein (His tag)
ACY1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Aminoacylase 1 (ACY1) (AA 1-408) protein (His tag)ACY1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Aminoacylase 1 (ACY1) (AA 1-408) protein (GST tag)ACY1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Aminoacylase 1 (ACY1) (Active) protein (His tag)ACY1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
Aminoacylase 1 (ACY1) protein (Myc-DYKDDDDK Tag)ACY1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
说明
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The tag is located at the C-terminal.
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限制
- 仅限研究用
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浓度
- 50 μg/mL
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缓冲液
- 50 mM Tris-HCl, pH 8.0, 100 mM glycine, 10 % glycerol.
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储存条件
- -80 °C
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储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Aminoacylase 1 (ACY1)
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别名
- aminoacylase 1
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背景
- This gene encodes a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and an acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. This gene is located on chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and this enzyme is the first member of a new family of zinc-binding enzymes. Mutations in this gene cause aminoacylase-1 deficiency, a metabolic disorder characterized by central nervous system defects and increased urinary excretion of N-acetylated amino acids. Alternative splicing of this gene results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ABHD14A (abhydrolase domain containing 14A) gene, as represented in GeneID:100526760. A related pseudogene has been identified on chromosome 18.
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分子量
- 45.7 kDa
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NCBI登录号
- NP_000657
抗原
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