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GOT1 Protein (AA 2-414) (His tag)
GOT1
宿主: 人
宿主: 大肠杆菌(E. Coli)
Recombinant
Greater than 95 % as determined by SEC-HPLC and reducing SDS-PAGE.
SDS, WB, ELISA, MS
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抗原
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GOT1
(Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))
蛋白类型
Recombinant
产品特性
AA 2-414
宿主
All origins for GOT1 蛋白
人
资源
All sources for GOT1 蛋白
大肠杆菌(E. Coli)
标记
This GOT1 protein is labelled with His tag.
应用范围
SDS-PAGE (SDS), Western Blotting (WB), ELISA, Mass Spectrometry (MS)
原理
Recombinant Human GOT1 Protein is produced by our E. coli expression system and the target gene encoding Ala2-Leu414 is expressed with a 6His tag at the C-terminus.
序列
MAPPSVFAEV PQAQPVLVFK LTADFREDPD PRKVNLGVGA YRTDDCHPWV LPVVKKVEQK IANDNSLNHE YLPILGLAEF RSCASRLALG DDSPALKEKR VGGVQSLGGT GALRIGADFL ARWYNGTNNK NTPVYVSSPT WENHNAVFSA AGFKDIRSYR YWDAEKRGLD LQGFLNDLEN APEFSIVVLH ACAHNPTGID PTPEQWKQIA SVMKHRFLFP FFDSAYQGFA SGNLERDAWA IRYFVSEGFE FFCAQSFSKN FGLYNERVGN LTVVGKEPES ILQVLSQMEK IVRITWSNPP AQGARIVAST LSNPELFEEW TGNVKTMADR ILTMRSELRA RLEALKTPGT WNHITDQIGM FSFTGLNPKQ VEYLVNEKHI YLLPSGRINV SGLTTKNLDY VATSIHEAVT KIQLEHHHHH H
纯度
Greater than 95 % as determined by SEC-HPLC and reducing SDS-PAGE.
过滤
0.2 μm filtered
内毒素水平
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
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使用细节
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限制
仅限研究用
贮存及处理
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状态
Liquid
缓冲液
Supplied as a 0.2 μM filtered solution of 20 mM Tris-HCl, 100 mM NaCl, 2 mM DTT, 20 % Glycerol, pH 7.5
注意事项
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
储存条件
-20 °C
储存方法
Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
目标详细情况
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抗原
GOT1
(Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))
别名
GOT1 (GOT1 产品 )
别名
ASTQTL1 Protein, GIG18 Protein, cAspAT Protein, cCAT Protein, AST Protein, ISSD Protein, NSD Protein, SD Protein, SIALIN Protein, SIASD Protein, SLD Protein, xr406 Protein, CASPAT Protein, ASPARTATE AMINOTRANSFERASE Protein, ATAAT Protein, MATERNAL EFFECT EMBRYO ARREST 17 Protein, MEE17 Protein, T26C19.9 Protein, T26C19_9 Protein, aspartate aminotransferase Protein, aatA Protein, 83.t00021 Protein, AI789014 Protein, Got-1 Protein, Aspat Protein, Gaspat Protein, AL022787 Protein, FABP-pm Protein, Got-2 Protein, mAspAT Protein, ASPATA Protein, glutamic-oxaloacetic transaminase 1 Protein, solute carrier family 17 member 5 Protein, glutamic-oxaloacetic transaminase 1 homeolog Protein, aspartate aminotransferase Protein, glutamic-oxaloacetic transaminase 1, soluble Protein, glutamic-oxaloacetic transaminase 2 Protein, glutamatic-oxaloacetic transaminase 2, mitochondrial Protein, GOT1 Protein, SLC17A5 Protein, got1.S Protein, AAT Protein, aspC Protein, EHI_006080 Protein, AST2 Protein, ast2 Protein, got1 Protein, Got1 Protein, GOT2 Protein, Got2 Protein
基因ID
2805
UniProt
P17174
途径
Hepatitis C , Monocarboxylic Acid Catabolic Process , Methionine Biosynthetic Process
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GOT1 蛋白
GOT1 Protein (AA 2-414) (His tag) (ABIN7312348)