Recombinant Human Ubiquitin-Conjugating Enzyme E2 B/UBE2B is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Met1-Ser152) of Human UBE2B fused with a polyhistidine tag at the C-terminus.
纯度
> 95 % as determined by reducing SDS-PAGE.
过滤
0.2 μm filtered
内毒素水平
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
缓冲液
Supplied as a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.2.
注意事项
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
储存条件
-80 °C
储存方法
Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
Ubiquitin-Conjugating Enzyme E2 B (UBE2B) is a member of the ubiquitin-conjugating enzyme family. UBE2B accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. It is shown that UBE2B interacts with RAD18, UBR2, and WAC. UBE2B is required for post-replicative DNA damage repair. Additional, UBE2B plays a role in sepsis-induced muscle protein proteolysis and cancer-induced cachexia. Alternative Names: Ubiquitin-Conjugating Enzyme E2 B, RAD6 Homolog B, HR6B, hHR6B, Ubiquitin Carrier Protein B, Ubiquitin-Conjugating Enzyme E2-17 kDa, Ubiquitin-Protein Ligase B, UBE2B, RAD6B