SERPINA1 Protein (AA 25-418) (His tag)
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- 抗原 See all SERPINA1 蛋白
- SERPINA1 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 25-418
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宿主
- 人
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资源
- 人细胞
- 标记
- This SERPINA1 protein is labelled with His tag.
- 原理
- Recombinant Human Serpin A1/alpha-1-Antitrypsin (C-6His)
- 序列
- EDPQGDAAQK TDTSHHDQDH PTFNKITPNL AEFAFSLYRQ LAHQSNSTNI FFSPVSIATA FAMLSLGTKA DTHDEILEGL NFNLTEIPEA QIHEGFQELL RTLNQPDSQL QLTTGNGLFL SEGLKLVDKF LEDVKKLYHS EAFTVNFGDT EEAKKQINDY VEKGTQGKIV DLVKELDRDT VFALVNYIFF KGKWERPFEV KDTEEEDFHV DQVTTVKVPM MKRLGMFNIQ HCKKLSSWVL LMKYLGNATA IFFLPDEGKL QHLENELTHD IITKFLENED RRSASLHLPK LSITGTYDLK SVLGQLGITK VFSNGADLSG VTEEAPLKLS KAVHKAVLTI DEKGTEAAGA MFLEAIPMSI PPEVKFNKPF VFLMIDQNTK SPLFMGKVVN PTQKVDHHHH HH
- 产品特性
- Recombinant Human Serpin A1 produced by transfected human cells is a secreted protein with sequence (Glu25-Lys418) of Human SERPIN A1 fused with a polyhistidine tag at the C-terminus.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product SERPINA1 蛋白
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serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1) (AA 26-134) protein (His-IF2DI Tag)
SERPINA1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1) (AA 1-418) protein (GST tag)SERPINA1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1) protein (His tag)SERPINA1 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by reducing SDS-PAGE.
serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1) protein (His tag)SERPINA1 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE
serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)SERPINA1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - 缓冲液
- Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl, 150 mM NaCl, 2 mM CaCl2, pH 7.5.
- 注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- 储存条件
- 4 °C/-20 °C/-80 °C
- 储存方法
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. - 有效期
- 3 months
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- 抗原
- SERPINA1 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1))
- 别名
- alpha-1-antitrypsin (SERPINA1 产品)
- 别名
- AACT Protein, ACT Protein, GIG25 Protein, A1AT Protein, PI Protein, alpha-1-PI Protein, A1A Protein, AAT Protein, PI1 Protein, PRO2275 Protein, alpha1AT Protein, Pi Protein, Spi1 Protein, MGC64429 Protein, serpina1d Protein, Aps Protein, SERPINA3-5 Protein, serpin family A member 3 Protein, alpha-1-antiproteinase F precursor Protein, serpin family A member 1 Protein, alpha-1-antitrypsin Protein, calumenin Protein, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 S homeolog Protein, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 Protein, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5 Protein, SH2B adaptor protein 2 Protein, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3 Protein, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 L homeolog Protein, SERPINA3 Protein, LOC100008973 Protein, SERPINA1 Protein, LOC100328621 Protein, CALU Protein, Serpina1 Protein, serpina1.S Protein, serpina1 Protein, SPIA5 Protein, Sh2b2 Protein, Calu Protein, serpina1.L Protein
- Sub Type
- Fusionprotein
- 背景
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Serpin A1 is a prototype member of the Serpin superfamily of the serine protease inhibitors. As one of the most abundant proteinase inhibitors in the circulation, it is synthesized in hepatocytes, and to a lesser extent, in macrophages as well as intestinal epithelial cell lines and secreted as the abundant proteinase inhibitor in the circulation whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Point mutations in the native SerpinA1 variants result in Serpin A1 deficiency, and consequently lead to several clinical complications such as pulmonary emphysema, juvenile hepatitis, cirrhosis, and hepatocellular carcinoma. For example, the Z variants (Glu342 to Lys) forms intracellular inclusion bodies, is not secreted, and leads to a severe SerpinA1 deficiency. Accordingly, Serpin A1 deficiency in circulation is associated with emphysema or liver disease.
Alternative Names: Alpha-1-Antitrypsin, Alpha-1 Protease Inhibitor, Alpha-1-Antiproteinase, Serpin A1, SERPINA1, AAT, PI - 分子量
- 45.35 kDa
- UniProt
- P01009
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