MMEL1 Protein (AA 63-765) (rho-1D4 tag)
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- 抗原 See all MMEL1 蛋白
- MMEL1 (Membrane Metallo-Endopeptidase-Like 1 (MMEL1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 63-765
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宿主
- 小鼠
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资源
- Insect Cells
- 标记
- This MMEL1 protein is labelled with rho-1D4 tag.
- 应用范围
- ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)
- 序列
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RALRDSSLKS DICTTPSCVI AAARILENMD QSRNPCENFY QYACGGWLRH HVIPETNSRY SVFDILRDEL EVILKGVLED STSQHRPAVE KAKTLYRSCM NQSVIEKRDS EPLLSVLKMV GGWPVAMDKW NETMGLKWEL ERQLAVLNSQ FNRRVLIDLF IWNDDQNSSR HVIYIDQPTL GMPSREYYFQ EDNNHKVRKA YLEFMTSVAT MLRKDQNLSK ESAMVREEMA EVLELETHLA NATVPQEKRH DVTALYHRMD LMELQERFGL KGFNWTLFIQ NVLSSVEVEL FPDEEVVVYG IPYLENLEDI IDSYSARTMQ NYLVWRLVLD RIGSLSQRFK EARVDYRKAL YGTTVEEVRW RECVSYVNSN MESAVGSLYI KRAFSKDSKS TVRELIEKIR SVFVDNLDEL NWMDEESKKK AQEKAMNIRE QIGYPDYILE DNNKHLDEEY SSLTFYEDLY FENGLQNLKN NAQRSLKKLR EKVDQNLWII GAAVVNAFYS PNRNQIVFPA GILQPPFFSK DQPQSLNFGG IGMVIGHEIT HGFDDNGRNF DKNGNMLDWW SNFSARHFQQ QSQCMIYQYG NFSWELADNQ NVNGFSTLGE NIADNGGVRQ AYKAYLRWLA DGGKDQRLPG LNLTYAQLFF INYAQVWCGS YRPEFAVQSI KTDVHSPLKY RVLGSLQNLP GFSEAFHCPR GSPMHPMKRC RIW
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Mmel1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin-free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product MMEL1 蛋白
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Membrane Metallo-Endopeptidase-Like 1 (MMEL1) (AA 1-779) protein (GST tag)
MMEL1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Membrane Metallo-Endopeptidase-Like 1 (MMEL1) (AA 1-779) protein (Strep Tag)Crystallography grade MMEL1 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Membrane Metallo-Endopeptidase-Like 1 (MMEL1) (AA 1-765) protein (Strep Tag)Crystallography grade MMEL1 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Membrane Metallo-Endopeptidase-Like 1 (MMEL1) (AA 49-779) protein (His tag)Crystallography grade MMEL1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Membrane Metallo-Endopeptidase-Like 1 (MMEL1) (AA 41-765) protein (His tag)Crystallography grade MMEL1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Membrane Metallo-Endopeptidase-Like 1 (MMEL1) (AA 74-779) protein (rho-1D4 tag)Crystallography grade MMEL1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- MMEL1 (Membrane Metallo-Endopeptidase-Like 1 (MMEL1))
- 别名
- Mmel1 (MMEL1 产品)
- 别名
- MMEL2 Protein, NEP2 Protein, NEPII Protein, NL1 Protein, NL2 Protein, SEP Protein, Mell1 Protein, Nl1 Protein, MMEL1 Protein, membrane metalloendopeptidase like 1 Protein, membrane metallo-endopeptidase-like 1 Protein, MMEL1 Protein, Mmel1 Protein, mmel1 Protein
- 背景
- Metalloprotease involved in sperm function, possibly by modulating the processes of fertilization and early embryonic development. Degrades a broad variety of small peptides with a preference for peptides shorter than 3 kDa containing neutral bulky aliphatic or aromatic amino acid residues. Shares the same substrate specificity with MME and cleaves peptides at the same amide bond. {ECO:0000269|PubMed:10542292, ECO:0000269|PubMed:11278416}.
- 分子量
- 82.9 kDa Including tag.
- UniProt
- Q9JLI3
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