SLC26A6 Protein (AA 1-758) (Strep Tag)
-
- 抗原 See all SLC26A6 蛋白
- SLC26A6 (Solute Carrier Family 26, Member 6 (SLC26A6))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-758
-
宿主
- 小鼠
-
资源
- Tobacco (Nicotiana tabacum)
- 标记
- This SLC26A6 protein is labelled with Strep Tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA
- 序列
-
MGLPDGSDQG THQTQALLSA AQEMELQRRD YHVERPLLNQ EQLEDLGHWG PAAKTHQWRT WFRCSRARAH SLLLQHVPVL GWLPRYPVRE WLLGDLLSGL SVAIMQLPQG LAYALLAGLP PMFGLYSSFY PVFIYFLFGT SRHISVGTFA VMSVMVGSVT ESLTADKAFV QGLNATADDA RVQVAYTLSF LVGLFQVGLG LVHFGFVVTY LSEPLVRSYT TAASVQVLVS QLKYVFGIKL SSHSGPLSVI YTVLEVCAQL PETVPGTVVT AIVAGVALVL VKLLNEKLHR RLPLPIPGEL LTLIGATGIS YGVKLNDRFK VDVVGNITTG LIPPVAPKTE LFATLVGNAF AIAVVGFAIA ISLGKIFALR HGYRVDSNQE LVALGLSNLI GGFFQCFPVS CSMSRSLVQE STGGNTQVAG AVSSLFILLI IVKLGELFRD LPKAVLAAVI IVNLKGMMKQ FSDICSLWKA NRVDLLIWLV TFVATILLNL DIGLAVSIVF SLLLVVVRMQ LPHYSVLGQV PDTDIYRDVA EYSGAKEVPG VKVFRSSATL YFANAELYSD SLKEKCGVDV DRLITQKKKR IKKQEMKLKR MKKAKKSQKQ DASSKISSVS VNVNTNLEDV KSNDVEGSEA KVHQGEELQD VVSSNQEDAK APTMTSLKSL GLPQPGFHSL ILDLSTLSFV DTVCIKSLKN IFRDFREIEV EVYIAACYSP VVAQLEAGHF FDESITKQHV FASVHDAVTF ALSHRKSVPK SPVLATKL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
-
Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
-
Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product SLC26A6 蛋白
-
Solute Carrier Family 26, Member 6 (SLC26A6) (AA 1-758) protein (GST tag)
SLC26A6 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Solute Carrier Family 26, Member 6 (SLC26A6) (AA 666-738) protein (GST tag)SLC26A6 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Solute Carrier Family 26, Member 6 (SLC26A6) (AA 1-759) protein (Strep Tag)Crystallography grade SLC26A6 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Solute Carrier Family 26, Member 6 (SLC26A6) (AA 1-115) protein (His tag)Crystallography grade SLC26A6 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Solute Carrier Family 26, Member 6 (SLC26A6) (AA 506-759) protein (His tag)Crystallography grade SLC26A6 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Solute Carrier Family 26, Member 6 (SLC26A6) (AA 1-117) protein (His tag)Crystallography grade SLC26A6 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Solute Carrier Family 26, Member 6 (SLC26A6) (AA 507-758) protein (His tag)Crystallography grade SLC26A6 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
-
- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
-
ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
-
- 抗原
- SLC26A6 (Solute Carrier Family 26, Member 6 (SLC26A6))
- 别名
- Slc26a6 (SLC26A6 产品)
- 别名
- B930010B04Rik Protein, CFEX Protein, Pat1 Protein, MGC81960 Protein, SLC26A6 Protein, slc26a6 Protein, MGC145603 Protein, si:dkey-20d21.8 Protein, solute carrier family 26 member 6 Protein, solute carrier family 26, member 6 Protein, solute carrier family 26 member 6 S homeolog Protein, anion exchanger SLC26A6 Protein, SLC26A6 Protein, Slc26a6 Protein, slc26a6.S Protein, slc26a6 Protein
- 背景
- Solute carrier family 26 member 6 (Anion exchange transporter) (Chloride-formate exchanger) (Pendrin-L1) (Pendrin-like protein 1) (Putative anion transporter-1) (Pat-1),FUNCTION: Apical membrane anion-exchanger with wide epithelial distribution that plays a role as a component of the pH buffering system for maintaining acid-base homeostasis. Acts as a versatile DIDS-sensitive inorganic and organic anion transporter that mediates the uptake of monovalent anions like chloride, bicarbonate, formate and hydroxyl ion and divalent anions like sulfate and oxalate. Functions in multiple exchange modes involving pairs of these anions, which include chloride-bicarbonate, chloride-oxalate, oxalate-formate, oxalate-sulfate and chloride-formate exchange. Apical membrane chloride-bicarbonate exchanger that mediates luminal chloride absorption and bicarbonate secretion by the small intestinal brush border membrane and contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption, possibly by providing a bicarbonate import pathway. Its association with carbonic anhydrase CA2 forms a bicarbonate transport metabolon, hence maximizes the local concentration of bicarbonate at the transporter site. Mediates also intestinal chloride absorption and oxalate secretion, thereby preventing hyperoxaluria and calcium oxalate urolithiasis. Transepithelial oxalate secretion, chloride-formate, chloride-oxalate and chloride-bicarbonate transport activities in the duodenum are inhibited by PKC activation in a calcium-independent manner. The apical membrane chloride-bicarbonate exchanger provides also a major route for fluid and bicarbonate secretion into the proximal tubules of the kidney as well as into the proximal part of the interlobular pancreatic ductal tree, where it mediates electrogenic chloride-bicarbonate exchange with a chloride-bicarbonate stoichiometry of 1:2, and hence will dilute and alkalinize protein-rich acinar secretion. Mediates also the transcellular sulfate absorption and oxalate secretion across the apical membrane in the duodenum and the formate ion efflux at the apical brush border of cells in the proximal tubules of kidney. Plays a role in sperm capacitation by increasing intracellular pH . {ECO:0000269|PubMed:11842009, ECO:0000269|PubMed:12119287, ECO:0000269|PubMed:16141316, ECO:0000269|PubMed:16532010, ECO:0000269|PubMed:16606687, ECO:0000269|PubMed:17053783, ECO:0000269|PubMed:17170027, ECO:0000269|PubMed:18046080, ECO:0000269|PubMed:18496516, ECO:0000269|PubMed:20150244, ECO:0000269|PubMed:20969732, ECO:0000269|PubMed:21976599, ECO:0000269|PubMed:22021714, ECO:0000269|PubMed:22895259, ECO:0000269|PubMed:23933580, ECO:0000269|PubMed:29530983}., FUNCTION: [Isoform 2]: Mediates electrogenic chloride-bicarbonate exchange with a chloride-bicarbonate stoichiometry of 1:2 (PubMed:12217875, PubMed:23933580). Also mediates exchange of chloride-formate and chloride-oxalate ions (PubMed:11459928, PubMed:12217875, PubMed:15203903, PubMed:17151144, PubMed:23933580). Mediates transcellular sulfate absorption (PubMed:12217875). {ECO:0000269|PubMed:11459928, ECO:0000269|PubMed:12217875, ECO:0000269|PubMed:15203903, ECO:0000269|PubMed:17151144, ECO:0000269|PubMed:23933580}.
- 分子量
- 82.8 kDa
- UniProt
- Q8CIW6
- 途径
- Dicarboxylic Acid Transport
-
