ROBO2 Protein (AA 22-1470) (rho-1D4 tag)
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- 抗原 See all ROBO2 蛋白
- ROBO2 (Roundabout, Axon Guidance Receptor, Homolog 2 (ROBO2))
- 蛋白类型
- Recombinant
- 产品特性
- AA 22-1470
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宿主
- 小鼠
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资源
- Insect Cells
- 标记
- This ROBO2 protein is labelled with rho-1D4 tag.
- 应用范围
- ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
- 序列
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SRLRQEDFPP RIVEHPSDVI VSKGEPTTLN CKAEGRPTPT IEWYKDGERV ETDKDDPRSH RMLLPSGSLF FLRIVHGRRS KPDEGSYVCV ARNYLGEAVS RNASLEVALL RDDFRQNPTD VVVAAGEPAI LECQPPRGHP EPTIYWKKDK VRIDDKEERI SIRGGKLMIS NTRKSDAGMY TCVGTNMVGE RDSDPAELTV FERPTFLRRP INQVVLEEEA VEFRCQVQGD PQPTVRWKKD DADLPRGRYD IKDDYTLRIK KAMSTDEGTY VCIAENRVGK VEASATLTVR VRPVAPPQFV VRPRDQIVAQ GRTVTFPCET KGNPQPAVFW QKEGSQNLLF PNQPQQPNSR CSVSPTGDLT ITNIQRSDAG YYICQALTVA GSILAKAQLE VTDVLTDRPP PIILQGPINQ TLAVDGTALL KCKATGEPLP VISWLKEGFT FLGRDPRATI QDQGTLQIKN LRISDTGTYT CVATSSSGET SWSAVLDVTE SGATISKNYD MNDLPGPPSK PQVTDVSKNS VTLSWQPGTP GVLPASAYII EAFSQSVSNS WQTVANHVKT TLYTVRGLRP NTIYLFMVRA INPQGLSDPS PMSDPVRTQD ISPPAQGVDH RQVQKELGDV VVRLHNPVVL TPTTVQVTWT VDRQPQFIQG YRVMYRQTSG LQASTVWQNL DAKVPTERSA VLVNLKKGVT YEIKVRPYFN EFQGMDSESK TVRTTEEAPS APPQSVTVLT VGSHNSTSIS VSWDPPPADH QNGIIQEYKI WCLGNETRFH INKTVDAAIR SVVIGGLFPG IQYRVEVAAS TSAGVGVKSE PQPIIIGGRN EVVITENNNS ITEQITDVVK QPAFIAGIGG ACWVILMGFS IWLYWRRKKR KGLSNYAVTF QRGDGGLMSN GSRPGLLNAG DPNYPWLADS WPATSLPVNN SNSGPNEIGN FGRGDVLPPV PGQGDKTATM LSDGAIYSSI DFTTKTTYNS SSQITQATPY ATTQILHSNS IHELAVDLPD PQWKSSVQQK TDLMGFGYSL PDQNKGNNGG KGGKKKKTKN SSKAQKNNGS TWANVPLPPP PVQPLPGTEL GHYAAEQENG YDSDSWCPPL PVQTYLHQGM EDELEEDEDR VPTPPVRGVA SSPAISFGQQ STATLTPSPR EEMQPMLQAH LDELTRAYQF DIAKQTWHIQ SNTPPPQPPA PPLGYVSGAL ISDLETDVPD EDADDEEEPL EIPRPLRALD QTPGSSMDNL DSSVTGKAFS SSQRQRPTSP FSTDSNTSAA QNQSQRPRPT KKHKGGRMDP QPVLPHRREG MPDDLPPPPD PPPGQGLRQQ IGLSQHSGNV ENSTERKGSS LERQQAANLE DTKSSLDCPA KTVLEWQRQT QDWINSTERQ EETRKAPHKQ GVGSEESLVP YSKPSFPSPG GHSSSGTSSS KGSTGPRKAD VLRGSHQRNA NDLLDIGYVG SNSQGQFTE
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Robo2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin-free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product ROBO2 蛋白
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Roundabout, Axon Guidance Receptor, Homolog 2 (ROBO2) (AA 441-540) protein (GST tag)
ROBO2 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Roundabout, Axon Guidance Receptor, Homolog 2 (ROBO2) (AA 881-1378) protein (His tag)Crystallography grade ROBO2 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Roundabout, Axon Guidance Receptor, Homolog 2 (ROBO2) (AA 885-1470) protein (His tag)Crystallography grade ROBO2 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Roundabout, Axon Guidance Receptor, Homolog 2 (ROBO2) (AA 22-859) protein (His tag)Crystallography grade ROBO2 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Roundabout, Axon Guidance Receptor, Homolog 2 (ROBO2) (AA 22-863) protein (His tag)Crystallography grade ROBO2 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Roundabout, Axon Guidance Receptor, Homolog 2 (ROBO2) (AA 22-1378) protein (rho-1D4 tag)Crystallography grade ROBO2 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Roundabout, Axon Guidance Receptor, Homolog 2 (ROBO2) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)ROBO2 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- ROBO2 (Roundabout, Axon Guidance Receptor, Homolog 2 (ROBO2))
- 别名
- Robo2 (ROBO2 产品)
- 别名
- SAX3 Protein, ROBO2 Protein, CG14347 Protein, CG14348 Protein, CG5481 Protein, CG5574 Protein, CT17326 Protein, D-Robo2 Protein, D-robo2 Protein, Dmel\\CG5481 Protein, Robo 2 Protein, Robo-2 Protein, Robo2 Protein, anon-EST:Liang-1.75 Protein, clone 1.75 Protein, dRobo-2 Protein, fus4 Protein, robo-2 Protein, robo2 Protein, unp1881 Protein, 2600013A04Rik Protein, 9430089E08Rik Protein, BB097918 Protein, D230004I22Rik Protein, mKIAA1568 Protein, sax3 Protein, roundabout guidance receptor 2 Protein, roundabout 2 Protein, roundabout, axon guidance receptor, homolog 2 (Drosophila) Protein, roundabout guidance receptor 2 S homeolog Protein, ROBO2 Protein, Robo2 Protein, robo2 Protein, robo2.S Protein
- 背景
- Receptor for SLIT2, and probably SLIT1, which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. {ECO:0000269|PubMed:15091338}.
- 分子量
- 160.0 kDa Including tag.
- UniProt
- Q7TPD3
- 途径
- Regulation of Cell Size
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