TRPV1 Protein (AA 1-839) (Strep Tag)
-
- 抗原 See all TRPV1 蛋白
- TRPV1 (Transient Receptor Potential Cation Channel, Subfamily V, Member 1 (TRPV1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-839
-
宿主
- 小鼠
-
资源
- Tobacco (Nicotiana tabacum)
- 标记
- This TRPV1 protein is labelled with Strep Tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA
- 序列
-
MEKWASLDSD ESEPPAQENS CPDPPDRDPN SKPPPAKPHI FATRSRTRLF GKGDSEEASP MDCPYEEGGL ASCPIITVSS VVTLQRSVDG PTCLRQTSQD SVSTGVETPP RLYDRRSIFD AVAQSNCQEL ESLLSFLQKS KKRLTDSEFK DPETGKTCLL KAMLNLHNGQ NDTIALLLDI ARKTDSLKQF VNASYTDSYY KGQTALHIAI ERRNMALVTL LVENGADVQA AANGDFFKKT KGRPGFYFGE LPLSLAACTN QLAIVKFLLQ NSWQPADISA RDSVGNTVLH ALVEVADNTA DNTKFVTNMY NEILILGAKL HPTLKLEELT NKKGLTPLAL AASSGKIGVL AYILQREIHE PECRHLSRKF TEWAYGPVHS SLYDLSCIDT CEKNSVLEVI AYSSSETPNR HDMLLVEPLN RLLQDKWDRF VKRIFYFNFF VYCLYMIIFT TAAYYRPVEG LPPYKLNNTV GDYFRVTGEI LSVSGGVYFF FRGIQYFLQR RPSLKSLFVD SYSEILFFVQ SLFMLVSVVL YFSHRKEYVA SMVFSLAMGW TNMLYYTRGF QQMGIYAVMI EKMILRDLCR FMFVYLVFLF GFSTAVVTLI EDGKNNSLPV ESPPHKCRGS ACRPGNSYNS LYSTCLELFK FTIGMGDLEF TENYDFKAVF IILLLAYVIL TYILLLNMLI ALMGETVNKI AQESKNIWKL QRAITILDTE KSFLKCMRKA FRSGKLLQVG FTPDGKDDFR WCFRVDEVNW TTWNTNVGII NEDPGNCEGV KRTLSFSLRS GRVSGRNWKN FALVPLLRDA STRDRHSTQP EEVQLKHYTG SLKPEDAEVF KDSMAPGEK
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
-
Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
-
Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product TRPV1 蛋白
-
Transient Receptor Potential Cation Channel, Subfamily V, Member 1 (TRPV1) (AA 21-124) protein (GST tag)
TRPV1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Transient Receptor Potential Cation Channel, Subfamily V, Member 1 (TRPV1) (AA 1-839) protein (Strep Tag)Crystallography grade TRPV1 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Transient Receptor Potential Cation Channel, Subfamily V, Member 1 (TRPV1) (AA 688-839) protein (His tag)Crystallography grade TRPV1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Transient Receptor Potential Cation Channel, Subfamily V, Member 1 (TRPV1) (AA 688-839) protein (His tag)Crystallography grade TRPV1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Transient Receptor Potential Cation Channel, Subfamily V, Member 1 (TRPV1) (AA 1-433) protein (His tag)Crystallography grade TRPV1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Transient Receptor Potential Cation Channel, Subfamily V, Member 1 (TRPV1) (AA 1-433) protein (His tag)Crystallography grade TRPV1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
-
- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
-
ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
-
- 抗原
- TRPV1 (Transient Receptor Potential Cation Channel, Subfamily V, Member 1 (TRPV1))
- 别名
- Trpv1 (TRPV1 产品)
- 别名
- si:dkey-9e15.2 Protein, VR.5'sv Protein, Vr1 Protein, Vr1l1 Protein, VR1 Protein, OTRPC1 Protein, TRPV1alpha Protein, TRPV1beta Protein, VR-1 Protein, transient receptor potential cation channel, subfamily V, member 1 Protein, transient receptor potential cation channel subfamily V member 1 Protein, trpv1 Protein, Trpv1 Protein, TRPV1 Protein
- 背景
- Transient receptor potential cation channel subfamily V member 1 (TrpV1) (Osm-9-like TRP channel 1) (OTRPC1) (Vanilloid receptor 1),FUNCTION: Ligand-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli (PubMed:15194687, PubMed:15489017). Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. Involved in mediation of inflammatory pain and hyperalgesia (PubMed:10764638). Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Activation by vanilloids, like capsaicin, and temperatures higher than 42 degrees Celsius, exhibits a time- and Ca(2+)-dependent outward rectification, followed by a long-lasting refractory state. Mild extracellular acidic pH (6.5) potentiates channel activation by noxious heat and vanilloids, whereas acidic conditions ( pH <6) directly activate the channel. Can be activated by endogenous compounds, including 12-hydroperoxytetraenoic acid and bradykinin. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accumbens by affecting AMPA receptors endocytosis (By similarity). {ECO:0000250|UniProtKB:O35433, ECO:0000269|PubMed:10764638, ECO:0000269|PubMed:15194687, ECO:0000269|PubMed:15489017}.
- 分子量
- 95.0 kDa
- UniProt
- Q704Y3
- 途径
- Dicarboxylic Acid Transport
-
