CD101 Protein (CD101) (AA 21-1033) (rho-1D4 tag)
-
- 抗原 See all CD101 蛋白
- CD101
- 蛋白类型
- Recombinant
- 产品特性
- AA 21-1033
-
宿主
- 小鼠
-
资源
- Insect Cells
- 标记
- This CD101 protein is labelled with rho-1D4 tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
-
QREVKIQEGP LYRAEGYPVS IRCTVSGHQG PSTQDFRWSI YLPSAPTKEV QIISTKDAGF SYAVYAQRVQ SKEIYIERLQ GDSVLLHISK LQMKDAGEYE CHTPNTDGKY FGSYSAKTNL TVVPDTLSAT MPSQTLSKKE GEPLELTCET TKATVQHTHL SLTWYLMQEG GGSQATEIVS LSKDFVLTPG SSYADRFVAG DVRLDKLGAT SFRLSVGKLQ PSDQGQVFCE ATEWIQDPDE TWTLITRKQT DQTALRIQPA ARDFTVSITA SSSPDEGKPL ELVCLAVGRD GNPQLQGVWF LNGKEIAQTD AGGVLDLKRD YRDRASQGQL QVSKLSAQTF SLKIFSVGPE DVGTYSCEVA EVARTQMGSW QVLQRKQSPG YRVQLREPAA RSVTVSAEQR TVWEGETLTL LCKAAGDVSA LSVSWWLTPQ DQSTPVFVAG MGQDGTVQLG VSSPGPAHRG NRRLEKVDWA TFRLEIASAM VTDSGTYECR VSERLQNQAK GLQSTQKISV TVKSLKSSLR VNLMSRQPQV MLAHTFHLSC VVRANYSDLK LPFSVTWQFQ PAGSGAFHRL IRIAHNGTVE WGDVLSQIHR KTKVSQSFFR SQLQIYDAAM EETGVYRCTV EVYDRDSICT SGPARVSATS NLLMITVTFP ESKLSVNSSS QVQELSISSS TQIECAILSR SAGNLPLSII WYFSSVSANA SYLKILEMDQ SSVVKYGDEF QTPRSKQKFY SEKVSQDLFL LNILSVEDSD QGHYHCAVEE WLLSTNDTWQ KLERKTSGLT ELKLRPTGSQ VHVSKVNWTG NATEYGEAGF SCSLDGSGST ASLYSVTWYR GRGTATATAA AVANATATIT APAGSQMLVH LQYDGLLQYG REGSRRLQHC YRSSPTDFVL KLHRVEMEDA GIYWCRVTEW QQHGHPGKWI NQASGESQRM VLRVLRSEPT VSSLICSSGP LLHFLIVCPF VMLLLLATSF LCLYRKARKL SQLSLSAKKE KALWVGMRKT SLQKEAGEES GHY
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
-
- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Cd101 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
-
Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin-free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product CD101 蛋白
-
CD101 (CD101) (AA 653-759) protein (GST tag)
CD101 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
CD101 (CD101) (AA 21-954) protein (His tag)Crystallography grade CD101 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
CD101 (CD101) (AA 21-970) protein (His tag)Crystallography grade CD101 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
CD101 (CD101) (AA 21-1021) protein (rho-1D4 tag)Crystallography grade CD101 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
-
- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
-
Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
-
- 抗原
- CD101
- 别名
- Cd101 (CD101 产品)
- 别名
- Gm1016 Protein, Gm734 Protein, Igsf2 Protein, EWI-101 Protein, IGSF2 Protein, V7 Protein, CD101 molecule Protein, CD101 antigen Protein, CD101 Protein, Cd101 Protein
- 背景
- Plays a role as inhibitor of T-cells proliferation induced by CD3. Inhibits expression of IL2RA on activated T-cells and secretion of IL2. Inhibits tyrosine kinases that are required for IL2 production and cellular proliferation. Inhibits phospholipase C-gamma-1/PLCG1 phosphorylation and subsequent CD3-induced changes in intracellular free calcium. Prevents nuclear translocation of nuclear factor of activated T-cell to the nucleus. Plays a role in the inhibition of T-cell proliferation via IL10 secretion by cutaneous dendritic cells (By similarity). {ECO:0000250}.
- 分子量
- 113.3 kDa Including tag.
- UniProt
- A8E0Y8
-
