c-MET Protein (AA 25-932) (His tag,AVI tag,Biotin)
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Quick Overview for c-MET Protein (AA 25-932) (His tag,AVI tag,Biotin) (ABIN3137684)
抗原
See all c-MET (MET) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 25-932
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标记
- This c-MET protein is labelled with His tag,AVI tag,Biotin.
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品牌
- MABSol® - PrecisionAvi
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序列
- AA 25-932
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特异性
- Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
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产品特性
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This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 104.3 kDa. The protein migrates as 40-45 kDa α subunit and 75 kDa β subunit under reducing (R) condition (SDS-PAGE) due to glycosylation.
This product is biotinylated with precision by using the Avitag™ technology, resulting in precise, single residue biotinylation at the lysine of the tag. This leads to minimal interence with protein binding, structure, and function, as well as uniform orientation upon immobilization. -
内毒素水平
- Less than 1.0 EU per μg by the LAL method.
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Met Proto-Oncogene (MET) (AA 25-932) protein (His-Avi Tag)
MET 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE, > 95 % as determined by HPLC
Met Proto-Oncogene (MET) (AA 25-930) (Active) protein (His tag)MET 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Met Proto-Oncogene (MET) (AA 25-932) (Active) protein (Biotin,His-Avi Tag)MET 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Met Proto-Oncogene (MET) (AA 956-1080) protein (His-IF2DI Tag)MET 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Met Proto-Oncogene (MET) (AA 26-125) protein (GST tag)MET 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Met Proto-Oncogene (MET) (AA 25-932) protein (His tag)MET 宿主: 人 宿主: Mammalian Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
Met Proto-Oncogene (MET) (AA 25-932) protein (Fc Tag)MET 宿主: 人 宿主: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Met Proto-Oncogene (MET) (Active) protein (His tag)MET 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active
Met Proto-Oncogene (MET) (AA 956-1390) (Active) protein (GST tag,His tag)MET 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90 % as determined by reducing SDS-PAGE. Active
Met Proto-Oncogene (MET) (Active) protein (Fc Tag)MET 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 92 % as determined by SDS-PAGE Active
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说明
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Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.
This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled. -
限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
- PBS, pH 7.4
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注意事项
- Please avoid repeated freeze-thaw cycles.
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储存条件
- -20 °C
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: "Fabs-in-tandem immunoglobulin is a novel and versatile bispecific design for engaging multiple therapeutic targets." in: mAbs, Vol. 9, Issue 7, pp. 1118-1128, (2018) (PubMed).
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: "Fabs-in-tandem immunoglobulin is a novel and versatile bispecific design for engaging multiple therapeutic targets." in: mAbs, Vol. 9, Issue 7, pp. 1118-1128, (2018) (PubMed).
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- c-MET (MET) (Met Proto-Oncogene (MET))
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别名
- HGFR
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背景
- Hepatocyte growth factor receptor (HGFR) is also known as mesenchymal-epithelial transition factor (MET), c-Met, and is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. HGFR protein possesses tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. HGFR is normally expressed by cells of epithelial origin, while expression of HGF is restricted to cells of mesenchymal origin. Upon HGF stimulation, HGFR induces several biological responses that collectively give rise to a program known as invasive growth. Abnormal HGFR activation in cancer correlates with poor prognosis, where aberrantly active HGFR triggers tumor growth, formation of new blood vessels (angiogenesis) that supply the tumor with nutrients, and cancer spread to other organs (metastasis). HGFR is deregulated in many types of human malignancies, including cancers of kidney, liver, stomach, breast, and brain. Normally, only stem cells and progenitor cells express HGFR, However, cancer stem cells are thought to hijack the ability of normal stem cells to express HGFR, and thus become the cause of cancer persistence and spread to other sites in the body. Various mutations in the HGFR gene are associated with papillary renal carcinoma. HGFR mediates a complex program known as invasive growth. Activation of HGFR triggers mitogenesis, and morphogenesis.
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分子量
- 104.3 kDa
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途径
- RTK signaling, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor
抗原
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