NR1H3 Protein (AA 1-445) (Strep Tag)
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- 抗原 See all NR1H3 蛋白
- NR1H3 (Nuclear Receptor Subfamily 1, Group H, Member 3 (NR1H3))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-445
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宿主
- 小鼠
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资源
- Tobacco (Nicotiana tabacum)
- 标记
- This NR1H3 protein is labelled with Strep Tag.
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB), ELISA
- 序列
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MSLWLEASMP DVSPDSATEL WKTEPQDAGD QGGNTCILRE EARMPQSTGV ALGIGLESAE PTALLPRAET LPEPTELRPQ KRKKGPAPKM LGNELCSVCG DKASGFHYNV LSCEGCKGFF RRSVIKGARY VCHSGGHCPM DTYMRRKCQE CRLRKCRQAG MREECVLSEE QIRLKKLKRQ EEEQAQATSV SPRVSSPPQV LPQLSPEQLG MIEKLVAAQQ QCNRRSFSDR LRVTPWPIAP DPQSREARQQ RFAHFTELAI VSVQEIVDFA KQLPGFLQLS REDQIALLKT SAIEVMLLET SRRYNPGSES ITFLKDFSYN REDFAKAGLQ VEFINPIFEF SRAMNELQLN DAEFALLIAI SIFSADRPNV QDQLQVERLQ HTYVEALHAY VSINHPHDPL MFPRMLMKLV SLRTLSSVHS EQVFALRLQD KKLPPLLSEI WDVHE
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product NR1H3 蛋白
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Nuclear Receptor Subfamily 1, Group H, Member 3 (NR1H3) (AA 1-387) protein (GST tag)
NR1H3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Nuclear Receptor Subfamily 1, Group H, Member 3 (NR1H3) (AA 1-447) protein (Strep Tag)Crystallography grade NR1H3 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Nuclear Receptor Subfamily 1, Group H, Member 3 (NR1H3) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)NR1H3 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Nuclear Receptor Subfamily 1, Group H, Member 3 (NR1H3) (AA 1-402), (Isoform 3) protein (His-SUMO Tag)NR1H3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- NR1H3 (Nuclear Receptor Subfamily 1, Group H, Member 3 (NR1H3))
- 别名
- Nr1h3 (NR1H3 产品)
- 别名
- NR1H3 Protein, LXR-a Protein, LXRA Protein, RLD-1 Protein, AU018371 Protein, LXR Protein, RLD1 Protein, Unr1 Protein, LXRalpha Protein, LXR-alpha Protein, im:7141239 Protein, wu:fd59b01 Protein, zgc:113339 Protein, zgc:158542 Protein, nuclear receptor subfamily 1 group H member 3 Protein, nuclear receptor subfamily 1, group H, member 3 Protein, NR1H3 Protein, nr1h3 Protein, Nr1h3 Protein
- 背景
- Oxysterols receptor LXR-alpha (Liver X receptor alpha) (Nuclear receptor subfamily 1 group H member 3),FUNCTION: Nuclear receptor that exhibits a ligand-dependent transcriptional activation activity (PubMed:18055760, PubMed:19520913, PubMed:20427281). Interaction with retinoic acid receptor (RXR) shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8. Interplays functionally with RORA for the regulation of genes involved in liver metabolism (By similarity). Induces LPCAT3-dependent phospholipid remodeling in endoplasmic reticulum (ER) membranes of hepatocytes, driving SREBF1 processing and lipogenesis (PubMed:28846071, PubMed:25806685). Via LPCAT3, triggers the incorporation of arachidonate into phosphatidylcholines of ER membranes, increasing membrane dynamics and enabling triacylglycerols transfer to nascent very low-density lipoprotein (VLDL) particles (PubMed:25806685). Via LPCAT3 also counteracts lipid-induced ER stress response and inflammation, likely by modulating SRC kinase membrane compartmentalization and limiting the synthesis of lipid inflammatory mediators (PubMed:24206663). {ECO:0000250|UniProtKB:Q13133, ECO:0000269|PubMed:18055760, ECO:0000269|PubMed:19520913, ECO:0000269|PubMed:20427281, ECO:0000269|PubMed:24206663, ECO:0000269|PubMed:25806685, ECO:0000269|PubMed:28846071}.
- 分子量
- 50.4 kDa
- UniProt
- Q9Z0Y9
- 途径
- Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Nuclear Hormone Receptor Binding, Cellular Response to Molecule of Bacterial Origin, Hepatitis C
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