RBM33 Protein (AA 2-1231) (His tag)
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- 抗原 See all RBM33 products
- RBM33 (RNA Binding Motif Protein 33 (RBM33))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-1231
- 宿主
- 小鼠
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资源
- Insect Cells
- 标记
- This RBM33 protein is labelled with His tag.
- 应用范围
- Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- 序列
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AAALGAGGGA GAGDDDFDQF DKPGAERSWR RRAADEDWDS ELEDDLLGED LLSGKKNQSD LSDEELNDDL LQSDNEEEEN FSSQGVTISL NTTSGIVTSF ELSDNTNDQS GEQESEYEQG DDELAYHKPE EQELYTQEYP EEGQYEGHDA ELTEDQIEYG DEPEEEQLYS DEVLDIEINE PLDEFTDEEY LQAYGGQQGL QVREDCEAED DLDEITDSQV ASETHEGGME TLELQKDIKE ESDEEDDDDE ESGRLRFKTE RKEGTIIRLS DVTRERRNIP ETLELSAEAK AALLEFEERE RQHKQGRYGS RRGGRRGGSL MCRGMGDQRR DNSERGRMKE HRPALLPTQP SVVAHSPRLI PPPQPQPPPP PPPPPPQQQP IRSLFQQQQL QPLLPLQHPH HPSPPQGVHM PPQIETPRMM LTPPPVTPQQ PKNIHINPHF KGTVVTPVQV PLLPVPSQPR PAVGPQRFPG PPEFPQHTPG PVPNSFNQPP RLPLQDQWRA PPPPQERDPF FLGVSGEPRF PSHLFLEQRS PPPPPPPPTL LNSSHPVPTQ SPLPFTQPGP AFNQQGQQPV FPRERPVRPA LQPPGPVGIL HFSQPGSATA RPFIPPRQPF LPSPGQPFLP THAQPNLQGP LHPPLPPPHQ PQPQPQQPQQ QPQHHQHQPP LQPPLQPPHQ PPPQHQPPPQ HQPQQHQHHH HLSAPPPPLM PMSQPQFRPH VQTAQPQPSS SRMQCTPHQG LRHNAASQNI SKRPMQQMQP TAPRNSNLRE LPIAPSHVLE MSGNRCSSTP VAQVKSIVNT SPPCRAVVSS RSSQGNTDAK AKPLSPEAQP KEEAKPEAEF PDEDEETRLY RLKIEEQKRL REEILKQKEL RRQQQAGARK KELLERLAQQ QQQQQQQQHQ PQQQQQQPQQ IYGSQTSMEQ EELAATPSPT NGNPLLPFPG AQCRQNVKTR LLVKNQDITT ASVQPKAVNF VPPGANVQHQ GQHLRPLKHL RQLPHKVLQV KPMDMEETPH SPQAARVTSL QGRPQDTKPG VKRTVMHRAN SGGGGDGPHV SSKVRVIKLS GGQGGESDGF SHTEGQPQRL PQPPDMRQQP TRKVTLTKGV PQQPQHLPVG PHMYPAIPPG IKSIQGIHPA KKAIMHGRGR GVAGPMGRGR LMPNKQNLRV VECKPQPCVV SVEGLSSSTT DVQLKSLLMS VGPIQSLQML PQQRKAIAKF KEPAHALAFQ QKFHRHMIDL SHINVALIVE
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Rbm33 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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