EIF4A3 Protein (AA 2-411) (His tag)
-
- 抗原 See all EIF4A3 蛋白
- EIF4A3 (Eukaryotic Translation Initiation Factor 4A3 (EIF4A3))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-411
-
宿主
- 小鼠
-
资源
- Insect Cells
- 标记
- This EIF4A3 protein is labelled with His tag.
- 应用范围
- ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)
- 序列
-
AANATMATSG SARKRLLKEE DMTKVEFETS EEVDVTPTFD TMGLREDLLR GIYAYGFEKP SAIQQRAIKQ IIKGRDVIAQ SQSGTGKTAT FSVSVLQCLD IQVRETQALI LAPTRELAVQ IQKGLLALGD YMNVQCHACI GGTNVGEDIR KLDYGQHVVA GTPGRVFDMI RRRSLRTRAI KMLVLDEADE MLNKGFKEQI YDVYRYLPPA TQVVLISATL PHEILEMTNK FMTDPIRILV KRDELTLEGI KQFFVAVERE EWKFDTLCDL YDTLTITQAV IFCNTKRKVD WLTEKMREAN FTVSSMHGDM PQKERESIMK EFRSGASRVL ISTDVWARGL DVPQVSLIIN YDLPNNRELY IHRIGRSGRY GRKGVAINFV KNDDIRILRD IEQYYSTQID EMPMNVADLI
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
-
- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Eif4a3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
-
Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product EIF4A3 蛋白
-
Eukaryotic Translation Initiation Factor 4A3 (EIF4A3) (AA 1-411) protein (His tag)
EIF4A3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Eukaryotic Translation Initiation Factor 4A3 (EIF4A3) (AA 1-411) protein (GST tag)EIF4A3 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Eukaryotic Translation Initiation Factor 4A3 (EIF4A3) (AA 312-411) protein (GST tag)EIF4A3 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Eukaryotic Translation Initiation Factor 4A3 (EIF4A3) (AA 1-411) protein (His tag)Crystallography grade EIF4A3 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Eukaryotic Translation Initiation Factor 4A3 (EIF4A3) (AA 2-411) protein (His tag)Crystallography grade EIF4A3 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Eukaryotic Translation Initiation Factor 4A3 (EIF4A3) (AA 1-411) protein (His tag)Crystallography grade EIF4A3 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Eukaryotic Translation Initiation Factor 4A3 (EIF4A3) protein (Myc-DYKDDDDK Tag)EIF4A3 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
-
Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
-
- 抗原
- EIF4A3 (Eukaryotic Translation Initiation Factor 4A3 (EIF4A3))
- 别名
- Eif4a3 (EIF4A3 产品)
- 背景
- ATP-dependent RNA helicase. Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH-RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH-RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes), specifically inhibits formation of proapoptotic, the function is different from the established EJC assembly (By similarity). Involved in craniofacial development (By similarity). {ECO:0000250}.
- 分子量
- 47.7 kDa Including tag.
- UniProt
- Q91VC3
-
