LNPEP Protein (AA 132-1025) (His tag)
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- 抗原 See all LNPEP 蛋白
- LNPEP (Leucyl/cystinyl Aminopeptidase (LNPEP))
- 蛋白类型
- Recombinant
- 产品特性
- AA 132-1025
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宿主
- 小鼠
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资源
- Insect Cells
- 标记
- This LNPEP protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
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PRCTFTKEGC HKTNQSAELI QPVATNGKVF PWAQIRLPTA IIPLCYELSL HPNLTSMTFR GSVTISLQAL QDTRDIILHS TGHNISRVTF MSAVSSQEKQ VEILEYPYHE QIAVVAPEPL LTGHNYTLKI EYSANISNSY YGFYGITYTD KSNEKKYFAA TQFEPLAARS AFPCFDEPAF KATFIIKITR NEHHTALSNM PKKSSVPAEE GLIQDEFSES VKMSTYLVAF IVGEMRNLSQ DVNGTLVSVY AVPEKIGQVH HALDTTIKLL EFYQTYFEIQ YPLKKLDLVA IPDFEAGAME NWGLLTFREE TLLYDNATSS VADRKLVTKI IAHELAHQWF GNLVTMQWWN DLWLNEGFAT FMEYFSVEKI FKELNSYEDF LDARFKTMRK DSLNSSHPIS SSVQSSEQIE EMFDSLSYFK GASLLLMLKS YLSEDVFRHA VILYLHNHSY AAIQSDDLWD SFNEVTDKTL DVKKMMKTWT LQKGFPLVTV QRKGTELLLQ QERFFLRMQP ESQPSDTSHL WHIPISYVTD GRNYSEYRSV SLLDKKSDVI NLTEQVQWVK VNSNMTGYYI VHYAHDDWTA LINQLKRNPY VLSDKDRANL INNIFELAGL GKVPLRMAFD LIDYLKNETH TAPITEALFQ TNLIYNLLEK LGHMDLSSRL VARVHKLLQN QIQQQTWTDE GTPSMRELRS ALLEFACAHS LENCTTMATN LFDSWMASNG TQSLPTDVMV TVFKVGARTE KGWLFLFSMY SSMGSEAEKN KILEALASSE DVHKLYWLMK SSLDGDIIRT QKLSLIIRTV GRHFPGHLLA WDFVKENWNK LVHKFHLGSY TIQSIVAGST HLFSTKTHLS EVQAFFENQS EATLKLRCVQ EALEVIQLNI QWMVRNLKTL SQWL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Lnpep Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product LNPEP 蛋白
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Leucyl/cystinyl Aminopeptidase (LNPEP) (AA 132-1025) protein (His tag)
Crystallography grade LNPEP 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Leucyl/cystinyl Aminopeptidase (LNPEP) (AA 926-1025) protein (GST tag)LNPEP 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Leucyl/cystinyl Aminopeptidase (LNPEP) (AA 1-1025) protein (Strep Tag)Crystallography grade LNPEP 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Leucyl/cystinyl Aminopeptidase (LNPEP) (AA 1-1025) protein (Strep Tag)Crystallography grade LNPEP 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Leucyl/cystinyl Aminopeptidase (LNPEP) (AA 1-110) protein (His tag)Crystallography grade LNPEP 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Leucyl/cystinyl Aminopeptidase (LNPEP) (AA 1-109) protein (His tag)Crystallography grade LNPEP 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Leucyl/cystinyl Aminopeptidase (LNPEP) (AA 155-1025) protein (rho-1D4 tag)Crystallography grade LNPEP 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Leucyl/cystinyl Aminopeptidase (LNPEP) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)LNPEP 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- LNPEP (Leucyl/cystinyl Aminopeptidase (LNPEP))
- 别名
- Lnpep (LNPEP 产品)
- 别名
- fb73h12 Protein, wu:fb73h12 Protein, zgc:66103 Protein, 2010309L07Rik Protein, 4732490P18Rik Protein, CAP Protein, IRAP Protein, PLAP Protein, gp160 Protein, vp165 Protein, P-LAP Protein, Vp165 Protein, LNPEP Protein, leucyl and cystinyl aminopeptidase Protein, leucyl/cystinyl aminopeptidase L homeolog Protein, leucyl/cystinyl aminopeptidase Protein, LNPEP Protein, lnpep.L Protein, lnpep Protein, Lnpep Protein
- 背景
- Release of an N-terminal amino acid, cleave before cysteine, leucine as well as other amino acids. Degrades peptide hormones such as oxytocin, vasopressin and angiotensin III, and plays a role in maintaining homeostasis during pregnancy. May be involved in the inactivation of neuronal peptides in the brain. Cleaves Met-enkephalin and dynorphin. Binds angiotensin IV and may be the angiotensin IV receptor in the brain (By similarity). {ECO:0000250}.
- 分子量
- 103.5 kDa Including tag.
- UniProt
- Q8C129
- 途径
- Regulation of long-term Neuronal Synaptic Plasticity
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