KCNC2 Protein (AA 478-642) (His tag)
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- 抗原 See all KCNC2 蛋白
- KCNC2 (Potassium Voltage-Gated Channel, Shaw-Related Subfamily, Member 2 (KCNC2))
- 蛋白类型
- Recombinant
- 产品特性
- AA 478-642
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宿主
- 小鼠
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资源
- Insect Cells
- 标记
- This KCNC2 protein is labelled with His tag.
- 应用范围
- ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)
- 序列
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NNFGMYYSLA MAKQKLPRKR KKHIPPAPLA SSPTFCKTEL NMACNSTQSD TCLGKENRLL EHNRSVLSGD DSTGSEPPLS PPERLPIRRS STRDKNRRGE TCFLLTTGDY TCASDGGIRK GYEKSRSLNN IAGLAGNALR LSPVTSPYNS PCPLRRSRSP IPSIL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Kcnc2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product KCNC2 蛋白
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Potassium Voltage-Gated Channel, Shaw-Related Subfamily, Member 2 (KCNC2) (AA 1-638) protein (Strep Tag)
Crystallography grade KCNC2 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
Potassium Voltage-Gated Channel, Shaw-Related Subfamily, Member 2 (KCNC2) (AA 1-642) protein (Strep Tag)Crystallography grade KCNC2 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
Potassium Voltage-Gated Channel, Shaw-Related Subfamily, Member 2 (KCNC2) (AA 1-229) protein (His tag)Crystallography grade KCNC2 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
Potassium Voltage-Gated Channel, Shaw-Related Subfamily, Member 2 (KCNC2) (AA 474-638) protein (His tag)Crystallography grade KCNC2 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
Potassium Voltage-Gated Channel, Shaw-Related Subfamily, Member 2 (KCNC2) (AA 1-233) protein (His tag)Crystallography grade KCNC2 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- KCNC2 (Potassium Voltage-Gated Channel, Shaw-Related Subfamily, Member 2 (KCNC2))
- 别名
- Kcnc2 (KCNC2 产品)
- 别名
- KV3.2 Protein, KShIIIA Protein, Kv3.2 Protein, AW047325 Protein, B230117I07 Protein, KCNC2 Protein, zgc:162956 Protein, potassium voltage-gated channel subfamily C member 2 Protein, potassium voltage gated channel, Shaw-related subfamily, member 2 Protein, potassium voltage-gated channel, Shaw-related subfamily, member 2 Protein, potassium channel, voltage gated Shaw related subfamily C, member 2 Protein, KCNC2 Protein, Kcnc2 Protein, kcnc2 Protein
- 背景
- Voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes, primarily in the brain. Contributes to the regulation of the fast action potential repolarization and in sustained high-frequency firing in neurons of the central nervous system (PubMed:10561420, PubMed:10414303, PubMed:11124984, PubMed:10903572, PubMed:11506885, PubMed:15317859, PubMed:15917463, PubMed:17761775, PubMed:21414897). Homotetramer channels mediate delayed-rectifier voltage-dependent potassium currents that activate rapidly at high-threshold voltages and inactivate slowly (PubMed:10414303). Forms tetrameric channels through which potassium ions pass in accordance with their electrochemical gradient. The channel alternates between opened and closed conformations in response to the voltage difference across the membrane (By similarity). Can form functional homotetrameric and heterotetrameric channels that contain variable proportions of KCNC1, and possibly other family members as well, channel properties depend on the type of alpha subunits that are part of the channel (PubMed:10531438, PubMed:12000114). Channel properties may be modulated by either the association with ancillary subunits, such as KCNE1, KCNE2 and KCNE3 or indirectly by nitric oxide (NO) through a cGMP- and PKG-mediated signaling cascade, slowing channel activation and deactivation of delayed rectifier potassium channels (By similarity). Contributes to fire sustained trains of very brief action potentials at high frequency in thalamocortical and suprachiasmatic nucleus (SCN) neurons, in hippocampal and neocortical interneurons and in retinal ganglion cells (PubMed:10561420, PubMed:10903572, PubMed:11506885, PubMed:17761775). Sustained maximal action potential firing frequency in inhibitory hippocampal interneurons is negatively modulated by histamine H2 receptor activation in a cAMP- and protein kinase (PKA) phosphorylation-dependent manner (PubMed:10903572). Plays a role in maintaining the fidelity of synaptic transmission in neocortical GABAergic interneurons by generating action potential (AP) repolarization at nerve terminals, thus reducing spike-evoked calcium influx and GABA neurotransmitter release (PubMed:15917463). Required for long-range synchronization of gamma oscillations over distance in the neocortex (PubMed:22539821). Contributes to the modulation of the circadian rhythm of spontaneous action potential firing in suprachiasmatic nucleus (SCN) neurons in a light-dependent manner (PubMed:21414897). {ECO:0000250|UniProtKB:P22462, ECO:0000269|PubMed:10531438, ECO:0000269|PubMed:10561420, ECO:0000269|PubMed:10903572, ECO:0000269|PubMed:11124984, ECO:0000269|PubMed:12000114, ECO:0000269|PubMed:15317859, ECO:0000269|PubMed:15917463, ECO:0000269|PubMed:17761775, ECO:0000269|PubMed:21414897, ECO:0000269|PubMed:22539821, ECO:0000305|PubMed:10414303, ECO:0000305|PubMed:11506885}.
- 分子量
- 19.1 kDa Including tag.
- UniProt
- Q14B80
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