Aurora A Protein (AA 1-395) (Strep Tag)
-
- 抗原 See all Aurora A (AURKA) 蛋白
- Aurora A (AURKA) (Aurora Kinase A (AURKA))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-395
-
宿主
- 小鼠
-
资源
- Tobacco (Nicotiana tabacum)
- 标记
- This Aurora A protein is labelled with Strep Tag.
- 应用范围
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- 序列
-
MDRCKENCVS RPVKTTVPFG PKRVLVTEQI PSQNLGSASS GQAQRVLCPS NSQRVPSQAQ KLGAGQKPAP KQLPAASVPR PVSRLNNPQK NEQPAASGND SEKEQASLQK TEDTKKRQWT LEDFDIGRPL GKGKFGNVYL ARERQSKFIL ALKVLFKTQL EKANVEHQLR REVEIQSHLR HPNILRLYGY FHDATRVYLI LEYAPLGTVY RELQKLSKFD EQRTATYITE LANALSYCHS KRVIHRDIKP ENLLLGSNGE LKIADFGWSV HAPSSRRTTM CGTLDYLPPE MIEGRMHDEK VDLWSLGVLC YEFLVGMPPF EAHTYQETYR RISRVEFTFP DFVTEGARDL ISRLLKHNAS QRLTLAEVLE HPWIKANSSK PPTGHTSKEP TSKSS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
-
Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
-
Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product AURKA 蛋白
-
Aurora Kinase A (AURKA) (AA 1-403) protein (GST tag)
AURKA 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP, KAA
Aurora Kinase A (AURKA) (AA 1-403) (Active) protein (GST tag)AURKA 宿主: 人 宿主: Insect cells (Sf21) Recombinant SDS, Func Active
Aurora Kinase A (AURKA) (AA 1-403) (Active) protein (His-GST)AURKA 宿主: 人 宿主: Insect cells (Sf9) Recombinant SDS, Func Active
Aurora Kinase A (AURKA) (AA 1-403) protein (His tag)AURKA 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
Aurora Kinase A (AURKA) (AA 1-110) protein (GST tag)AURKA 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Aurora Kinase A (AURKA) (AA 1-100) protein (GST tag)AURKA 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Aurora Kinase A (AURKA) (AA 1-403) protein (Strep Tag)Crystallography grade AURKA 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
-
- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
-
ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
-
- 抗原
- Aurora A (AURKA) (Aurora Kinase A (AURKA))
- 别名
- Aurka (AURKA 产品)
- 别名
- AIK Protein, ARK1 Protein, AURA Protein, AURORA2 Protein, BTAK Protein, PPP1R47 Protein, STK15 Protein, STK6 Protein, STK7 Protein, AIRK1 Protein, AU019385 Protein, AW539821 Protein, Ark1 Protein, Aurora-A Protein, Ayk1 Protein, IAK Protein, IAK1 Protein, Stk6 Protein, 87A7-9/2 Protein, Aur-A Protein, AurA Protein, CG3068 Protein, Dmel\\CG3068 Protein, aur-A Protein, aurA Protein, ck10 Protein, ck[10] Protein, l(3)87Ac Protein, l(3)ck10 Protein, ARK-1 Protein, Eg2 Protein, aik Protein, ark1 Protein, aur-a Protein, aura Protein, aurka-a Protein, aurora2 Protein, btak Protein, stk15 Protein, stk6 Protein, stk7 Protein, pEg2 Protein, wu:fb37c10 Protein, wu:fc02c02 Protein, zgc:100912 Protein, AURKA Protein, aurka Protein, aurora kinase A Protein, aurora A Protein, aurora kinase A L homeolog Protein, hypothetical protein Protein, aurora kinase A S homeolog Protein, AURKA Protein, Aurka Protein, aurA Protein, aurka.L Protein, aurka Protein, Aurora-A Protein, aurka.S Protein
- 背景
- Aurora kinase A (EC 2.7.11.1) (Aurora 2) (Aurora/IPL1-related kinase 1) (ARK-1) (Aurora-related kinase 1) (Ipl1- and aurora-related kinase 1) (Serine/threonine-protein kinase 15) (Serine/threonine-protein kinase 6) (Serine/threonine-protein kinase Ayk1) (Serine/threonine-protein kinase aurora-A),FUNCTION: Mitotic serine/threonine kinase that contributes to the regulation of cell cycle progression (By similarity). Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis (PubMed:9245792, PubMed:19075002). Required for normal spindle positioning during mitosis and for the localization of NUMA1 and DCTN1 to the cell cortex during metaphase (By similarity). Required for initial activation of CDK1 at centrosomes (By similarity). Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2 (By similarity). Regulates KIF2A tubulin depolymerase activity (By similarity). Required for normal axon formation (By similarity). Plays a role in microtubule remodeling during neurite extension (PubMed:19668197). Important for microtubule formation and/or stabilization (By similarity). Also acts as a key regulatory component of the p53/TP53 pathway, and particularly the checkpoint-response pathways critical for oncogenic transformation of cells, by phosphorylating and destabilizing p53/TP53 (By similarity). Phosphorylates its own inhibitors, the protein phosphatase type 1 (PP1) isoforms, to inhibit their activity (By similarity). Inhibits cilia outgrowth (By similarity). Required for cilia disassembly via phosphorylation of HDAC6 and subsequent deacetylation of alpha-tubulin (PubMed:20643351). Regulates protein levels of the anti-apoptosis protein BIRC5 by suppressing the expression of the SCF(FBXL7) E3 ubiquitin-protein ligase substrate adapter FBXL7 through the phosphorylation of the transcription factor FOXP1 (By similarity). {ECO:0000250|UniProtKB:A0A8I3S724, ECO:0000250|UniProtKB:O14965, ECO:0000269|PubMed:19075002, ECO:0000269|PubMed:19668197, ECO:0000269|PubMed:20643351, ECO:0000269|PubMed:9245792}.
- 分子量
- 44.8 kDa
- UniProt
- P97477
- 途径
- Cell Division Cycle, Asymmetric Protein Localization
-
