HSD17B8 Protein (AA 1-259) (Strep Tag)
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- 抗原 See all HSD17B8 蛋白
- HSD17B8 (Hydroxysteroid (17-Beta) Dehydrogenase 8 (HSD17B8))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-259
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宿主
- 小鼠
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资源
- Tobacco (Nicotiana tabacum)
- 标记
- This HSD17B8 protein is labelled with Strep Tag.
- 应用范围
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- 序列
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MASQLRLRSA LALVTGAGSG IGRAISVRLA AEGAAVAACD LDGAAAQDTV RLLGSPGSED GAPRGKHAAF QADVSQGPAA RRLLEEVQAC FSRPPSVVVS CAGITRDEFL LHMSEEDWDR VIAVNLKGTF LVTQAAAQAL VSSGGRGSII NISSIIGKVG NIGQTNYASS KAGVIGLTQT AARELGRHGI RCNSVLPGFI ATPMTQKMPE KVKDKVTAMI PLGHMGDPED VADVVAFLAS EDSGYITGAS VEVSGGLFM
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product HSD17B8 蛋白
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Hydroxysteroid (17-Beta) Dehydrogenase 8 (HSD17B8) (AA 1-261) protein (His tag)
HSD17B8 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Hydroxysteroid (17-Beta) Dehydrogenase 8 (HSD17B8) (AA 1-261) protein (GST tag)HSD17B8 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Hydroxysteroid (17-Beta) Dehydrogenase 8 (HSD17B8) (AA 28-127) protein (GST tag)HSD17B8 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Hydroxysteroid (17-Beta) Dehydrogenase 8 (HSD17B8) (AA 1-261) protein (Strep Tag)Crystallography grade HSD17B8 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Hydroxysteroid (17-Beta) Dehydrogenase 8 (HSD17B8) protein (Myc-DYKDDDDK Tag)HSD17B8 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- HSD17B8 (Hydroxysteroid (17-Beta) Dehydrogenase 8 (HSD17B8))
- 别名
- Hsd17b8 (HSD17B8 产品)
- 别名
- KE6 Protein, fabgl Protein, zgc:112550 Protein, si:dz12f11.4 Protein, HKE6 Protein, FABGL Protein, ke6 Protein, fabg Protein, hke6 Protein, ring2 Protein, h2-ke6 Protein, d6s2245e Protein, D17H6S112E Protein, H-2Ke6 Protein, Hsd17b8 Protein, Ke-6 Protein, Ke6 Protein, Ring2 Protein, D6S2245E Protein, FABG Protein, H2-KE6 Protein, RING2 Protein, SDR30C1 Protein, dJ1033B10.9 Protein, Fabgl Protein, hydroxysteroid (17-beta) dehydrogenase 8 Protein, hydroxysteroid 17-beta dehydrogenase 8 Protein, H2-K region expressed gene 6 Protein, hsd17b8 Protein, HSD17B8 Protein, H2-Ke6 Protein, Hsd17b8 Protein
- 背景
- (3R)-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.n12) (17-beta-hydroxysteroid dehydrogenase 8) (17-beta-HSD 8) (3-ketoacyl-[acyl-carrier-protein] reductase alpha subunit) (KAR alpha subunit) (3-oxoacyl-[acyl-carrier-protein] reductase) (Estradiol 17-beta-dehydrogenase 8) (EC 1.1.1.62) (Protein Ke6) (Ke-6) (Testosterone 17-beta-dehydrogenase 8) (EC 1.1.1.239),FUNCTION: Required for the solubility and assembly of the heterotetramer 3-ketoacyl-[acyl carrier protein] (ACP) reductase functional complex (KAR or KAR1) that forms part of the mitochondrial fatty acid synthase (mtFAS). Alpha-subunit of the KAR complex, acts as scaffold protein, required for the stability of carbonyl reductase type-4 (CBR4, beta-subunit of the KAR complex) and for its 3-ketoacyl-ACP reductase activity, thereby participating in mitochondrial fatty acid biosynthesis. Catalyzes the NAD-dependent conversion of (3R)-3-hydroxyacyl-CoA into 3-ketoacyl-CoA (3-oxoacyl-CoA) with no chain length preference, this enzymatic activity is not needed for the KAR function. Prefers (3R)-3-hydroxyacyl-CoA over (3S)-3-hydroxyacyl-CoA and displays enzymatic activity only in the presence of NAD(+)(H). Cooperates with enoyl-CoA hydratase 1 in mitochondria, together they constitute an alternative route to the auxiliary enzyme pathways for the breakdown of Z-PUFA (cis polyunsaturated fatty acid) enoyl-esters (By similarity). NAD-dependent 17-beta-hydroxysteroid dehydrogenase with highest activity towards estradiol. It efficiently catalyzes the oxidation of estradiol (E2), testosterone, and dihydrotestosterone. Primarily an oxidative enzyme, it can switch to a reductive mode determined in the appropriate physiologic milieu and catalyze the reduction of estrone (E1) to form biologically active estradiol (E2) (PubMed:9712896). {ECO:0000250|UniProtKB:Q92506, ECO:0000269|PubMed:9712896}.
- 分子量
- 26.6 kDa
- UniProt
- P50171
- 途径
- Steroid Hormone Biosynthesis
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