MMP 9 Protein (AA 108-730) (His tag)
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- 抗原 See all MMP 9 (MMP9) 蛋白
- MMP 9 (MMP9) (Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))
- 蛋白类型
- Recombinant
- 产品特性
- AA 108-730
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宿主
- 小鼠
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资源
- Insect Cells
- 标记
- This MMP 9 protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
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FQTFKGLKWD HHNITYWIQN YSEDLPRDMI DDAFARAFAV WGEVAPLTFT RVYGPEADIV IQFGVAEHGD GYPFDGKDGL LAHAFPPGAG VQGDAHFDDD ELWSLGKGVV IPTYYGNSNG APCHFPFTFE GRSYSACTTD GRNDGTPWCS TTADYDKDGK FGFCPSERLY TEHGNGEGKP CVFPFIFEGR SYSACTTKGR SDGYRWCATT ANYDQDKLYG FCPTRVDATV VGGNSAGELC VFPFVFLGKQ YSSCTSDGRR DGRLWCATTS NFDTDKKWGF CPDQGYSLFL VAAHEFGHAL GLDHSSVPEA LMYPLYSYLE GFPLNKDDID GIQYLYGRGS KPDPRPPATT TTEPQPTAPP TMCPTIPPTA YPTVGPTVGP TGAPSPGPTS SPSPGPTGAP SPGPTAPPTA GSSEASTESL SPADNPCNVD VFDAIAEIQG ALHFFKDGWY WKFLNHRGSP LQGPFLTART WPALPATLDS AFEDPQTKRV FFFSGRQMWV YTGKTVLGPR SLDKLGLGPE VTHVSGLLPR RLGKALLFSK GRVWRFDLKS QKVDPQSVIR VDKEFSGVPW NSHDIFQYQD KAYFCHGKFF WRVSFQNEVN KVDHEVNQVD DVGYVTYDLL QCP
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Mmp9 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product MMP9 蛋白
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Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9) protein (His-Avi Tag,Biotin)
MMP9 宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9) protein (His-Avi Tag)MMP9 宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9) (AA 20-706) protein (His tag)MMP9 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9) (AA 20-730) protein (His tag)MMP9 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9) (AA 532-707) protein (His tag)MMP9 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9) (AA 1-707) protein (GST tag)MMP9 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9) (Active) protein (His tag)MMP9 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9) proteinMMP9 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE
Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9) (AA 226-391) protein (His tag)MMP9 宿主: 大鼠 宿主: Yeast (Pichia pastoris) Recombinant > 95 % WB, SDS, ELISA, IP
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- MMP 9 (MMP9) (Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))
- 别名
- Mmp9 (MMP9 产品)
- 别名
- CLG4B Protein, GELB Protein, MANDP2 Protein, MMP-9 Protein, AW743869 Protein, B/MMP9 Protein, Clg4b Protein, pro-MMP-9 Protein, mmp-9 Protein, fgMMP-9 Protein, mmp9 Protein, ZFMMP-9 Protein, wu:fb02g06 Protein, wu:fb07b05 Protein, wu:fi98c09 Protein, wu:fj05a08 Protein, zgc:64165 Protein, clg4b Protein, gelb Protein, mandp2 Protein, matrix metallopeptidase 9 Protein, collagenase Protein, matrix metalloproteinase 9 Protein, matrix metallopeptidase 9 S homeolog Protein, MMP9 Protein, RB3913 Protein, BPSS0666 Protein, Sbal_3732 Protein, Bcer98_0486 Protein, Shew185_0630 Protein, Shal_3056 Protein, Sbal195_0657 Protein, Lbys_3550 Protein, Palpr_2084 Protein, Mmp9 Protein, mmp9 Protein, mmp9.S Protein
- 背景
- Could play a role in bone osteoclastic resorption. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments (By similarity). {ECO:0000250}.
- 分子量
- 69.6 kDa Including tag.
- UniProt
- P41245
- 途径
- Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events
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