Moesin Protein (MSN) (AA 2-577) (His tag)
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- 抗原 See all Moesin (MSN) 蛋白
- Moesin (MSN)
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-577
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宿主
- 小鼠
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资源
- 大肠杆菌（E. Coli）
- 标记
- This Moesin protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
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PKTISVRVTT MDAELEFAIQ PNTTGKQLFD QVVKTIGLRE VWFFGLQYQD TKAFSTWLKL NKKVTAQDVR KESPLLFKFR AKFYPEDVSE ELIQDITQRL FFLQVKEGIL NDDIYCPPET AVLLASYAVQ SKYGDFNKEV HKSGYLAGDK LLPQRVLEQH KLNKDQWEER IQVWHEEHRG MLREDAVLEY LKIAQDLEMY GVNYFSIKNK KGSELWLGVD ALGLNIYEQN DRLTPKIGFP WSEIRNISFN DKKFVIKPID KKAPDFVFYA PRLRINKRIL ALCMGNHELY MRRRKPDTIE VQQMKAQARE EKHQKQMERA LLENEKKKRE LAEKEKEKIE REKEELMEKL KQIEEQTKKA QQELEEQTRR ALELEQERKR AQSEAEKLAK ERQEAEEAKE ALLQASRDQK KTQEQLASEM AELTARISQL EMARKKKESE AVEWQQKAQM VQEDLEKTRA ELKTAMSTPH VAEPAENEHD EQDENGAEAS AELRADAMAK DRSEEERTTE AEKNERVQKH LKALTSELAN ARDESKKTAN DMIHAENMRL GRDKYKTLRQ IRQGNTKQRI DEFESM
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Msn Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product MSN 蛋白
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Moesin (MSN) (AA 1-577) protein (GST tag)
MSN 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Moesin (MSN) (AA 410-577) protein (His-IF2DI Tag)MSN 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Moesin (MSN) (AA 422-531) protein (GST tag)MSN 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Moesin (MSN) (AA 1-577) protein (His tag)MSN 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Moesin (MSN) (AA 2-577) protein (His tag)Crystallography grade MSN 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Moesin (MSN) protein (Myc-DYKDDDDK Tag)MSN 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- Moesin (MSN)
- 别名
- Msn (MSN 产品)
- 别名
- CG10701 Protein, D17 Protein, Dmel\\CG10701 Protein, Dmoe Protein, EMR1 Protein, ERM Protein, Emr1 Protein, anon-WO03040301.155 Protein, anon-WO03040301.157 Protein, anon-WO03040301.159 Protein, anon-WO03040301.161 Protein, dMoe Protein, gma Protein, l(1)G0067 Protein, l(1)G0323 Protein, l(1)G0404 Protein, l(1)G0415 Protein, moe Protein, moesin/ezrin/radixin homolog mRNA Protein, cb567 Protein, rdx Protein, wu:fb14b03 Protein, zgc:85640 Protein, MSN Protein, C78546 Protein, moesin Protein, Moesin Protein, moesin a Protein, moesin L homeolog Protein, MSN Protein, Moe Protein, msna Protein, msn.L Protein, moes Protein, Msn Protein
- 背景
- Probably involved in connections of major cytoskeletal structures to the plasma membrane.
- 分子量
- 68.6 kDa Including tag.
- UniProt
- P26041
- 途径
- Asymmetric Protein Localization
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