HCK Protein (AA 2-524) (His tag)
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- 抗原 See all HCK 蛋白
- HCK (Hemopoietic Cell Kinase (HCK))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-524
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宿主
- 小鼠
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资源
- Insect Cells
- 标记
- This HCK protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
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GGRSSCEDPG CPRSEGRAPR MGCVKSRFLR DGSKASKTEP SANQKGPVYV PDPTSSSKLG PNNSNSMPPG FVEGSEDTIV VALYDYEAIH REDLSFQKGD QMVVLEEAGE WWKARSLATK KEGYIPSNYV ARVNSLETEE WFFKGISRKD AERHLLAPGN MLGSFMIRDS ETTKGSYSLS VRDFDPQHGD TVKHYKIRTL DSGGFYISPR STFSSLQELV LHYKKGKDGL CQKLSVPCVS PKPQKPWEKD AWEIPRESLQ MEKKLGAGQF GEVWMATYNK HTKVAVKTMK PGSMSVEAFL AEANLMKSLQ HDKLVKLHAV VSQEPIFIVT EFMAKGSLLD FLKSEEGSKQ PLPKLIDFSA QISEGMAFIE QRNYIHRDLR AANILVSASL VCKIADFGLA RIIEDNEYTA REGAKFPIKW TAPEAINFGS FTIKSDVWSF GILLMEIVTY GRIPYPGMSN PEVIRALEHG YRMPRPDNCP EELYNIMIRC WKNRPEERPT FEYIQSVLDD FYTATESQYQ QQP
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Hck Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product HCK 蛋白
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Hemopoietic Cell Kinase (HCK) (AA 1-505) (Active) protein (His-GST)
HCK 宿主: 人 宿主: Insect cells (Sf9) Recombinant SDS, Func Active
Hemopoietic Cell Kinase (HCK) (AA 1-505) protein (GST tag)HCK 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Hemopoietic Cell Kinase (HCK) (AA 1-80) protein (GST tag)HCK 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Hemopoietic Cell Kinase (HCK) (AA 2-526) protein (His tag)Crystallography grade HCK 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Hemopoietic Cell Kinase (HCK) protein (Myc-DYKDDDDK Tag)HCK 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- HCK (Hemopoietic Cell Kinase (HCK))
- 别名
- Hck (HCK 产品)
- 别名
- laloo Protein, HCK Protein, MGC107870 Protein, JTK9 Protein, p59Hck Protein, p61Hck Protein, AI849071 Protein, Bmk Protein, Hctk Protein, p56Hck Protein, HCK proto-oncogene, Src family tyrosine kinase L homeolog Protein, HCK proto-oncogene, Src family tyrosine kinase Protein, hemopoietic cell kinase Protein, hck.L Protein, HCK Protein, hck Protein, Hck Protein
- 背景
- Non-receptor tyrosine-protein kinase found in hematopoietic cells that transmits signals from cell surface receptors and plays an important role in the regulation of innate immune responses, including neutrophil, monocyte, macrophage and mast cell functions, phagocytosis, cell survival and proliferation, cell adhesion and migration. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as FCGR1A and FCGR2A, but also CSF3R, PLAUR, the receptors for IFNG, IL2, IL6 and IL8, and integrins, such as ITGB1 and ITGB2. During the phagocytic process, mediates mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase to bring about the respiratory burst. Plays a role in the release of inflammatory molecules. Promotes reorganization of the actin cytoskeleton and actin polymerization, formation of podosomes and cell protrusions. Inhibits TP73-mediated transcription activation and TP73-mediated apoptosis. Phosphorylates CBL in response to activation of immunoglobulin gamma Fc region receptors. Phosphorylates ADAM15, BCR, ELMO1, FCGR2A, GAB1, GAB2, RAPGEF1, STAT5B, TP73, VAV1 and WAS (By similarity). {ECO:0000250, ECO:0000269|PubMed:10547366, ECO:0000269|PubMed:10799548, ECO:0000269|PubMed:12235133, ECO:0000269|PubMed:14551197, ECO:0000269|PubMed:16809022, ECO:0000269|PubMed:17513616, ECO:0000269|PubMed:18246197, ECO:0000269|PubMed:18625913, ECO:0000269|PubMed:19897576, ECO:0000269|PubMed:19903482, ECO:0000269|PubMed:8125254, ECO:0000269|PubMed:9400828, ECO:0000269|PubMed:9916742}.
- 分子量
- 60.0 kDa Including tag.
- UniProt
- P08103
- 途径
- Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling
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