COL15A1 Protein (AA 32-1367) (His tag)
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- 抗原 See all COL15A1 蛋白
- COL15A1 (Collagen, Type XV, alpha 1 (COL15A1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 32-1367
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宿主
- 小鼠
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资源
- Insect Cells
- 标记
- This COL15A1 protein is labelled with His tag.
- 应用范围
- ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
- 序列
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ATGSASQVHL DLTVLIGVPL PSSVSFTTGY GGFPAYSFGP GANVGRPART LIPPTFFRDF AIGVAVKPNS AQGGVLFAIT DAFQKVIYLG LRLSSVEDGR QRVILYYTEP GSHVSREAAV FSVPVMTNRW NRFAVTVQGE EVALFMDCEE QSQVRFQRSS WPLTFEPSAG IFVGNAGAMG LERFTGSIQQ LTIYSDPRTP EELCEAQESS ASGEASGFQE MDEVAEIMEA VTYTQAPPKE SHVDPISVPP TSSSPAEDSE LSGEPVPEGT PETNLSIIGH SSPEQGSGEI LNDTLEVHAM DGDPGTDDGS GDGALLNVTD GQGLSATATG EASVPVTTVL EAENGSMPTG SPTLAMFTQS IREVDTPDPE NLTTTASGDG EVPTSTDGDT EADSSPTGGP TLKPREEATL GSHGEEWLTP AVSKMPLKAF EEEEASGTAI DSLDVIFTPT VVLEQVSRRP TDIQATFTPT VVLEETSGAP TDTQDALTPT VAPEQMFTAE PTDGGDLVAS TEEAEEEGSG SMPPSGPPLP TPTVTPKRQV TLVGVEAEGS GPVGGLDEGS GSGDIVGNED LLRGPPGPPG PPGSPGIPGK PGTDVFMGPP GSPGEDGAPG EPGPQGPEGQ PGLDGASGQQ GMKGEKGARG PNGSAGEKGD PGNRGLPGPP GKNGEVGTPG VMGPPGPPGP PGPPGPGCTT ELGFEIEGSG DVRLLSKPTI SGPTSPSGPK GEKGEQGAKG ERGADGTSTM GPPGPRGPPG HVEVLSSSLI NITNGSMNFS DIPELMGPPG PDGVPGLPGF PGPRGPKGDT GVPGFPGLKG EQGEKGEPGA ILTGDVPLEM MKGRKGEPGI HGAPGPMGPK GPPGHKGEFG LPGRPGRPGL NGLKGAKGDR GVTLPGPPGL PGPPGPPGPP GAVVNIKGAV FPIPARPHCK TPVGTAHPGD PELVTFHGVK GEKGSWGLPG SKGEKGDQGA QGPPGPPVDP AYLRHFLNSL KGENEDASFR GESSNNLFVS GPPGLPGYPG LVGQKGEAVV GPQGPPGIPG LPGPPGFGRP GVPGPPGPPG PPGPPAILGA AVALPGPPGP PGQPGLPGSR NLVTALSDMG DMLQKAHLVI EGTFIYLRDS GEFFIRVRDG WKKLQLGELI PIPADSPPPP ALSSNPYQPQ PPLNPILSAN YERPVLHLVA LNTPVAGDIR ADFQCFQQAR AAGLLSTFRA FLSSHLQDLS TVVRKAERFG LPIVNLKGQV LFNNWDSIFS GDGGQFNTHI PIYSFDGRDV MTDPSWPQKV VWHGSNPHGV RLVDKYCEAW RTTDMAVTGF ASPLSTGKIL DQKAYSCANR LIVLCIENSF MTDTRK
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Col15a1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product COL15A1 蛋白
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Collagen, Type XV, alpha 1 (COL15A1) (AA 1212-1388) protein (His tag)
COL15A1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
Collagen, Type XV, alpha 1 (COL15A1) (AA 1198-1386) protein (His tag)Crystallography grade COL15A1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Collagen, Type XV, alpha 1 (COL15A1) (AA 1207-1386) protein (His tag)Crystallography grade COL15A1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Collagen, Type XV, alpha 1 (COL15A1) (AA 1213-1386) protein (His tag)Crystallography grade COL15A1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Collagen, Type XV, alpha 1 (COL15A1) (AA 1221-1386) protein (His tag)Crystallography grade COL15A1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Collagen, Type XV, alpha 1 (COL15A1) (AA 1177-1365) protein (His tag)Crystallography grade COL15A1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Collagen, Type XV, alpha 1 (COL15A1) (AA 28-1388) protein (His tag)Crystallography grade COL15A1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Collagen, Type XV, alpha 1 (COL15A1) protein (Myc-DYKDDDDK Tag)COL15A1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- COL15A1 (Collagen, Type XV, alpha 1 (COL15A1))
- 别名
- Col15a1 (COL15A1 产品)
- 别名
- COL15A1 Protein, col115a1 Protein, col15a1 Protein, col15a1l Protein, sb:cb605 Protein, collagen type XV alpha 1 chain Protein, collagen, type XV, alpha 1a Protein, collagen, type XV, alpha 1 Protein, COL15A1 Protein, col15a1a Protein, Col15a1 Protein
- 背景
- Structural protein that stabilizes microvessels and muscle cells, both in heart and in skeletal muscle., Restin potently inhibits angiogenesis.
- 分子量
- 137.9 kDa Including tag.
- UniProt
- O35206
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