DLD Protein (AA 36-509) (His tag)
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- 抗原 See all DLD 蛋白
- DLD (Dihydrolipoamide Dehydrogenase (DLD))
- 蛋白类型
- Recombinant
- 产品特性
- AA 36-509
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宿主
- 小鼠
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资源
- Insect Cells
- 标记
- This DLD protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
- 序列
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ADQPIEADVT VIGSGPGGYV AAIKSAQLGF KTVCIEKNET LGGTCLNVGC IPSKALLNNS HYYHMAHGKD FASRGIEIPE VRLNLEKMME QKHSAVKALT GGIAHLFKQN KVVHVNGFGK ITGKNQVTAT KADGSTQVID TKNILVATGS EVTPFPGITI DEDTIVSSTG ALSLKKVPEK LVVIGAGVIG VELGSVWQRL GADVTAVEFL GHVGGIGIDM EISKNFQRIL QRQGFKFKLN TKVTGATKKS DGKIDVSVEA ASGGKAEVIT CDVLLVCIGR RPFTQNLGLE ELGIELDPKG RIPVNNRFQT KIPNIYAIGD VVAGPMLAHK AEDEGIICVE GMAGGAVHID YNCVPSVIYT HPEVAWVGKS EEQLKEEGIE FKIGKFPFAA NSRAKTNADT DGMVKILGHK STDRVLGAHI LGPGAGEMVN EAALALEYGA SCEDIARVCH AHPTLSEAFR EANLAAAFGK PINF
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Dld Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product DLD 蛋白
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Dihydrolipoamide Dehydrogenase (DLD) (AA 36-509) protein (His tag)
DLD 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
Dihydrolipoamide Dehydrogenase (DLD) (AA 1-509) protein (GST tag)DLD 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Dihydrolipoamide Dehydrogenase (DLD) (AA 36-509) protein (His tag)Crystallography grade DLD 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Dihydrolipoamide Dehydrogenase (DLD) proteinDLD 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS, WB, ELISA, IP
Dihydrolipoamide Dehydrogenase (DLD) protein (Myc-DYKDDDDK Tag)DLD 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- DLD (Dihydrolipoamide Dehydrogenase (DLD))
- 别名
- Dld (DLD 产品)
- 别名
- DLDD Protein, DLDH Protein, E3 Protein, GCSL Protein, LAD Protein, PHE3 Protein, AI315664 Protein, AI746344 Protein, wu:fb24b05 Protein, DLD Protein, DDBDRAFT_0183800 Protein, DDBDRAFT_0216232 Protein, DDB_0183800 Protein, DDB_0216232 Protein, sc:d0402 Protein, dihydrolipoamide dehydrogenase Protein, dihydrolipoyl dehydrogenase Protein, deltaD Protein, DLD Protein, Dld Protein, dldh Protein, AT4G16155 Protein, CND05840 Protein, bfmBC Protein, GCSL Protein, LACBIDRAFT_182385 Protein, UREG_06178 Protein, lpd Protein, TAGG_RS02070 Protein, Arnit_2606 Protein, Mesil_1945 Protein, Trad_2118 Protein, Acear_0640 Protein, Fbal_0372 Protein, Ilyop_1890 Protein, Ftrac_1733 Protein, Ocepr_1753 Protein, Intca_2017 Protein, Deima_0504 Protein, dld Protein
- 背景
- Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.
- 分子量
- 51.2 kDa Including tag.
- UniProt
- O08749
- 途径
- Ribonucleoside Biosynthetic Process, Cell RedoxHomeostasis
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