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OTUD4 Protein (AA 1-1107) (Strep Tag)

Crystallography grade OTUD4 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
产品编号 ABIN3131014
发货至: 中国
  • 抗原 See all OTUD4 蛋白
    OTUD4 (OTU Domain Containing 4 (OTUD4))
    蛋白类型
    Recombinant
    产品特性
    AA 1-1107
    宿主
    小鼠
    资源
    • 1
    • 1
    Tobacco (Nicotiana tabacum)
    标记
    This OTUD4 protein is labelled with Strep Tag.
    应用范围
    ELISA, Western Blotting (WB), SDS-PAGE (SDS)
    序列
    MEAAVGAPDG VDQGGVGPLE DETPMDAYLR KLGLYRKLVA KDGSCLFRAV AEQVLHSQSR HVEVRMACIR YLRENREKFE AFIEGSFEEY LKRLENPQEW VGQVEISALS LMYRKDFVIY QEPNVSPSHV TENNFPEKVL LCFSNGNHYD IVYPITYKDS SAMCQSLLYE LLYEKVFKTD VSKIMMGLEA SEVAEESNSE ISDSEDDSCK SKSTAATDVN GFKPSGSENP KNNGNSADLP LSRKVLKSLN PAVYRNVEYE IWLKSKQAQQ KRDYSIAAGL QYEVGDKCHQ VRLDHNGKLS NADIHGVHSE NGLVLSEELG KKHTPKNLKP PPPESWNTVS GKKMKKPNSG QNFHSDTDYR GPKNLNKPIK APSALPPRLQ HPSSGVRQHA FSSHSTGSQS QKSSSEHKNL SRMPSQITRK PDRERAEDFD HVSRESYYFG LSPEERREKQ AIEESRLLYE IQNRDEQAFP ALSSSSVSQS PSQNSNACVP RKSSHARDRK GSMRRADAEE RKDKDSLRGH THVDKKPEPS TLEISDDKCT RVSSPSKSKK ECPSPVEQKP AEHIPLSNPA PLLVSPEVHL TPAVPSLPAT VPAWPSEPTT FGPTGVPAQI PILSVTQTTG PDAAVSQAHL TPSPVPVSIQ AVNQPLMPLP QTMSLYQDPL YPGFPCSEKG DRAIAPPYSL CQTGEDLPKD KNILRFFFNL GVKAYSCPMW APHSYLYPLH QAYMAACRMY PKVPVPVYPQ NTWFQEAPPA QSESDCPCTD AHYSLHPEAS VNGQMPQAEM GPPAFASPLV IPPSQVSEGH GQLSYQPELE SENPGQLLHA EYEESLSGKN MYPQQSFGPN PFLGPVPIAP PFFPHVWYGY PFQGFVENPV MRQNIVLPPD DKGELDLPLE NLDLSKECDS VSAVDEFPDA RVEGAHSLSA ASVSSKHEGR VEQSSQTRKA DIDLASGSSA VEGKGHPPTQ ILNREREPGS AEPEPKRTIQ SLKEKPEKVK DPKTAADVVS PGANSVDRLQ RPKEESSEDE NEVSNILRSG RSKQFYNQTY GSRKYKSDWG SSGRGGYQHV RGEESWKGQP NRSRDEGYQY HRHVRGRPYR GDRRRSGMGD GHRGQHT
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    产品特性
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    Expression System:

    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
    • We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
    1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    内毒素水平
    Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
    质量等级
    Crystallography grade
    Top Product
    Discover our top product OTUD4 蛋白
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    说明

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    OTUD4 (OTU Domain Containing 4 (OTUD4))
    别名
    Otud4 (OTUD4 产品)
    别名
    DUBA6 Protein, HIN1 Protein, HSHIN1 Protein, 4930431L18Rik Protein, AI449692 Protein, D8Ertd69e Protein, mKIAA1046 Protein, RGD1305606 Protein, OTU deubiquitinase 4 Protein, OTU domain containing 4 Protein, OTUD4 Protein, Otud4 Protein
    背景
    OTU domain-containing protein 4 (EC 3.4.19.12),FUNCTION: Deubiquitinase which hydrolyzes the isopeptide bond between the ubiquitin C-terminus and the lysine epsilon-amino group of the target protein. May negatively regulate inflammatory and pathogen recognition signaling in innate immune response. Upon phosphorylation at Ser-202 and Ser-204 residues, via IL-1 receptor and Toll-like receptor signaling pathway, specifically deubiquitinates 'Lys-63'-polyubiquitinated MYD88 adapter protein triggering down-regulation of NF-kappa-B-dependent transcription of inflammatory mediators (PubMed:29395066). Independently of the catalytic activity, acts as a scaffold for alternative deubiquitinases to assemble specific deubiquitinase-substrate complexes. Associates with USP7 and USP9X deubiquitinases to stabilize alkylation repair enzyme ALKBH3, thereby promoting the repair of alkylated DNA lesions (By similarity). {ECO:0000250|UniProtKB:Q01804, ECO:0000269|PubMed:29395066}.
    分子量
    123.1 kDa
    UniProt
    B2RRE7
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