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TTLL6 Protein (AA 1-822) (Strep Tag)

Crystallography grade TTLL6 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
产品编号 ABIN3130891
发货至: 中国
  • 抗原 See all TTLL6 蛋白
    TTLL6 (Tubulin tyrosine Ligase-Like Family, Member 6 (TTLL6))
    蛋白类型
    Recombinant
    产品特性
    AA 1-822
    宿主
    小鼠
    资源
    • 1
    • 1
    Tobacco (Nicotiana tabacum)
    标记
    This TTLL6 protein is labelled with Strep Tag.
    应用范围
    ELISA, Western Blotting (WB), SDS-PAGE (SDS)
    序列
    MLQCLTSESE EGAEEREESS TEDLEELKEF VTLAFVRENT QKRLQNAQQH GKKKRKKKRL VINLSNCRYD SVRRAAQQYG LREAGDNDDW TLYWTDYSVS LERVMEMKSY QKINHFPGMS EICRKDLLAR NMSRMLKLFP KDFHFFPRTW CLPADWGDLQ TYSRTRKNKT YICKPDSGCQ GRGIFITRSV KEIKPGEDMI CQLYISKPFI IDGFKFDLRV YVLVTSCDPL RVFVYNEGLA RFATTSYSHP NLDNLDEICM HLTNYSINKH SSNFVQDAFS GSKRKLSTFN SYMKTHGYDV EQIWRGIEDV IIKTLISAHP VIKHNYHTCF PSHTLNSACF EILGFDILLD RKLKPWLLEV NHSPSFSTDS KLDKEVKDSL LYDALVLINL GNCDKKKVLE EERQRGRFLQ QCPNREIRLE EVKGFQAMRL QKTEEYEKKN CGGFRLIYPG LNLEKYDKFF QDNSSLFQNT VASRARELYA RQLIQELRQK QEKKVFLKKA RKEETQGESA GEQARDKVVR LQRQRQQPKC KTVATCPPKQ SLHPVTLVSC TSGLLLNIRG LKKGEISESL EQKDTKEAML IPCKPVSARN YSSVPDLRSA NPSCFEPEFH VPNAKVKEVK SAFMVNIEST AQPITSVESS RDATAPISTS LESLASMSLS TSPECSSPES VHMVSYNHKQ QKASFHKPMQ EKKSKPLMFS KSRHLDLNCT SMKNDINRQY LMSEILQKVQ MKKKRPLFPA PKSQYPTLSK ERCPHSRSSS RKKEMNSPSV FVLQASHSRA ESLNDLLVVA TQARLDPRPS RSHSGTTTRD SSTQDPKHTA TA
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    产品特性
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    Expression System:

    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
    • We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
    1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    内毒素水平
    Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
    质量等级
    Crystallography grade
    Top Product
    Discover our top product TTLL6 蛋白
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    说明

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    TTLL6 (Tubulin tyrosine Ligase-Like Family, Member 6 (TTLL6))
    别名
    Ttll6 (TTLL6 产品)
    别名
    fc39g05 Protein, wu:fc39g05 Protein, TTL.6 Protein, 4932418K24Rik Protein, D11Moh43e Protein, D11Moh44e Protein, t8130b59 Protein, tubulin tyrosine ligase-like family, member 6 Protein, tubulin tyrosine ligase like 6 Protein, ttll6 Protein, TTLL6 Protein, Ttll6 Protein
    背景
    Tubulin polyglutamylase TTLL6 (EC 6.3.2.-) (Protein polyglutamylase TTLL6) (Tubulin--tyrosine ligase-like protein 6),FUNCTION: Polyglutamylase which modifies both tubulin and non-tubulin proteins, generating alpha-linked polyglutamate side chains on the gamma-carboxyl group of specific glutamate residues of target proteins (PubMed:17499049, PubMed:21074048, PubMed:20530212, PubMed:26829768, PubMed:32747782). Preferentially mediates ATP-dependent long polyglutamate chain elongation over the initiation step of the polyglutamylation reaction (PubMed:17499049, PubMed:21074048, PubMed:20530212, PubMed:26829768, PubMed:32747782). Preferentially modifies the alpha-tubulin tail over a beta-tail (PubMed:17499049, PubMed:20530212, PubMed:21074048, PubMed:32747782). Promotes tubulin polyglutamylation which stimulates spastin/SPAST-mediated microtubule severing, thereby regulating microtubule functions (PubMed:20530212). Mediates microtubule polyglutamylation in primary cilia axoneme which is important for ciliary structural formation and motility (PubMed:22246503). Mediates microtubule polyglutamylation in motile cilia, necessary for the regulation of ciliary coordinated beating (PubMed:23897886). Polyglutamylates non-tubulin protein nucleotidyltransferase CGAS, leading to CGAS DNA-binding inhibition, thereby preventing antiviral defense response (PubMed:26829768). {ECO:0000269|PubMed:17499049, ECO:0000269|PubMed:20530212, ECO:0000269|PubMed:21074048, ECO:0000269|PubMed:22246503, ECO:0000269|PubMed:23897886, ECO:0000269|PubMed:26829768, ECO:0000269|PubMed:32747782}.
    分子量
    94.5 kDa
    UniProt
    A4Q9E8
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